IFITM1 Rabbit Polyclonal Antibody
Catalog# ER2001-46
IFITM1 Rabbit Polyclonal Antibody
-
WB
-
Human
-
unconjugated
Overview
Product Name
IFITM1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human IFITM1 aa 1-50.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 14 kDa
Positive Control
K-562 cell lysate, HL-60 cell lysate, 293T cell lysate, A549 cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry and SARS-CoV S protein-mediated viral entry. Also implicated in cell adhesion and control of cell growth and migration. Plays a key role in the antiproliferative action of IFN-gamma either by inhibiting the ERK activation or by arresting cell growth in G1 phase in a p53-dependent manner. Acts as a positive regulator of osteoblast differentiation.
Background References
1. Gómez-Herranz M. et. al. The effects of IFITM1 and IFITM3 gene deletion on IFNγ stimulated protein synthesis. Cell Signal. 2019 Aug
2. Yang J. et. al. Combination of IFITM1 knockdown and radiotherapy inhibits the growth of oral cancer. Cancer Sci. 2018 Oct
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
9-27 antibody
CD 225 antibody
CD 225 antigen antibody
CD225 antibody
CD225 antigen antibody
Dispanin subfamily A member 2a antibody
DSPA2a antibody
IFI 17 antibody
IFI17 antibody
IFITM1 antibody
ExpandImages
-
Western blot analysis of IFITM1 on different lysates with Rabbit anti-IFITM1 antibody (ER2001-46) at 1/1,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: HL-60 cell lysate
Lane 3: 293T cell lysate
Lane 4: A549 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-46) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
