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ID2 Mouse Monoclonal Antibody [A4-D4]

Usd: 350

Specification

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Catalog# M1301-2

ID2 Mouse Monoclonal Antibody [A4-D4]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

ID2 Mouse Monoclonal Antibody [A4-D4]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein with Human ID2 aa 1-80 / 134.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 15 kDa

Positive Control

HEK-293 cell lysate, HT-29 cell lysate, SK-OV-3 cell lysate, human pancreatic cancer tissue.

Conjugation

unconjugated

Clone Number

A4-D4

RRID

AB_3073053

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2b

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:10,000

  • IHC-P

  • 1:100

Target

Function

DNA-binding protein inhibitor ID-2, is an inhibitor of differentiation protein, which, along with ID1, ID3, and ID4, plays a key role in the regulation of lineage commitment, cell fate decisions, and in the timing of differentiation during neurogenesis, lymphopoiesis, and neovascularization (angiogenesis). ID-2 may be an inhibitor of tissue-specific gene expression.

Background References

1. "Nucleo-cytoplasmic shuttling of Id2, a negative regulator of basic helix-loop-helix transcription factors."Kurooka H., Yokota Y.J. Biol. Chem. 280:4313-4320(2005)

2. "The mouse Id2 and Id4 genes: structural organization and chromosomal localization."Mantani A., Hernandez M.-C., Kuo W.-L., Israel M.A.Gene 222:229-235(1998)

Tissue Specificity

Highly expressed in early fetal tissues, including those of the central nervous system.

Subcellular Location

Cytoplasm, nucleus.

UNIPROT

SwissProt: Q02363 Human

SwissProt: P41136 Mouse

Synonyms

bHLHb26 antibody

Cell growth inhibiting gene 8 antibody

class B basic helix loop helix protein 26 antibody

Class B basic helix-loop-helix protein 26 antibody

DNA binding protein inhibitor ID 2 antibody

DNA binding protein inhibitor ID2 antibody

DNA-binding protein inhibitor ID-2 antibody

GIG 8 antibody

GIG8 antibody

Helix loop helix protein ID2 antibody

Expand

Images

  • Western blot analysis of ID2 on different lysates with Mouse anti-ID2 antibody (<a href="/products/M1301-2" style="font-weight: bold;text-decoration: underline;">M1301-2</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: HT-29 cell lysate<br />Lane 3: SK-OV-3 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 15 kDa<br />Observed band size: 15 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/M1301-2" style="font-weight: bold;text-decoration: underline;">M1301-2</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ID2 on different lysates with Mouse anti-ID2 antibody (M1301-2) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: HT-29 cell lysate
    Lane 3: SK-OV-3 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1301-2) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis on recombinant protein using anti-ID2 Mouse mAb (Cat. # <a href="/products/M1301-2" style="font-weight: bold;text-decoration: underline;">M1301-2</a>).

    Western blot analysis on recombinant protein using anti-ID2 Mouse mAb (Cat. # M1301-2).

  • Immunohistochemical analysis of paraffin- embedded human pancreatic cancer tissue using anti-ID2 Mouse mAb (Cat. # <a href="/products/M1301-2" style="font-weight: bold;text-decoration: underline;">M1301-2</a>).

    Immunohistochemical analysis of paraffin- embedded human pancreatic cancer tissue using anti-ID2 Mouse mAb (Cat. # M1301-2).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation