Senescence Associated Secretory Phenotype (SASP) Antibody Kit
Catalog# HAK21006
Senescence Associated Secretory Phenotype (SASP) Antibody Kit
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WB
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Human
Overview
Kit Components
| Product Includes | Specification | Application | Reactivity | Mw |
|---|---|---|---|---|
| IL-1 beta[HA601036] | 20µl | WB,ELISA,IHC-P,IF-Tissue | Human,Mouse,Rat | Predicted band size: 31 kDa |
| RANTES[ET1705-70] | 20µl | WB,IHC-P,IF-Cell,IF-Tissue | Human,Mouse | Predicted band size: 10 kDa |
| IP10[ET1704-27] | 20µl | WB,IF-Cell,IF-Tissue,IHC-P | Human | Predicted band size: 11 kDa |
| PAI1[HA601152] | 20µl | WB,IHC-P,IF-Tissue,FC | Human,Mouse,Rat | Predicted band size: 45 kDa |
| IL-6[R1412-2] | 20µl | WB,IF-Cell,IHC-P,FC | Human | Predicted band size: 24 kDa |
| TNF alpha[HA722022] | 20µl | WB | Human,Mouse | Predicted band size: 26 kDa |
| MMP-3[ET1705-98] | 20µl | WB,IHC-P | Human,Mouse,Rat | Predicted band size: 54 kDa |
| CCL2 / MCP-1[HA500042] | 20µl | WB,IHC-P,IF-Cell | Human | Predicted band size: 11 kDa |
| Goat Anti-Rabbit IgG (H+L)[HA1001] | 100µl | WB,ELISA,IHC-P | Rabbit | |
| Goat Anti-Mouse IgG (H+L)[HA1006] | 100µl | WB,ELISA,IHC-P | Mouse |
Product Description
Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit provides an economical means of detecting multiple components of the SASP. The kit includes enough antibody to perform two western blot experiments with each primary antibody.
Product Features
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Background
Senescence is characterized by stable stress-induced proliferative arrest and resistance to mitogenic stimuli, as well as the secretion of proteins such as cytokines, growth factors and proteases. These secreted proteins comprise the senescence-associated secretory phenotype (SASP). Senescent cells are thought to accumulate as an organism ages, and contribute to age-related diseases, including cancer, through promotion of inflammation and disruption of normal cellular function. The composition of the SASP varies, and SASP components can be either beneficial or deleterious in human disease, depending on the context. Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit provides a collection of antibodies to various SASP components, including TNF-alpha, interleukin-6 (IL-6), the multifunctional cytokine IL-1beta, the chemokines CXCL10, RANTES/CCL5 and MCP-1, the matrix metalloprotease MMP3, and the serine-protease inhibitor PAI-1.
Data Links
Background References
1. Tchkonia, T. et al. (2013) J Clin Invest 123, 966-72.
2. Sun, Y. et al. (2018) Trends Mol Med 24, 871-885.
3. Rao, S.G. and Jackson, J.G. (2016) Trends Cancer 2, 676-687.
Images
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Western blot analysis of IL-1 beta on different lysates with Rabbit anti-IL-1 beta antibody (ET1701-39) at 1/1,000 dilution.
Lane 1: THP-1 whole cell lysate
Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 25 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-39) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of PAI1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500124, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat brain tissue lysate
Lane 2: Rat cerebellum tissue lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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