Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Target
Function
Anti-Mouse IgG3(Fcγ fragment specific), AlpSdAbs® VHH(HRP) is designed for detecting the Fc region of mouse IgG3. Anti-Mouse IgG3(Fcγ fragment specific), AlpSdAbs® VHH(HRP) is based on monovalent, recombinant single domain antibodies to mouse IgG3(Fcγ fragment specific) coupled to HRP, and the Anti-Mouse IgG3(Fcγ fragment specific), AlpSdAbs® VHH(HRP) detects the Fc region of mouse IgG3 selectively, no reactivity with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins., ·High lot-to-lot consistency·Increased sensitivity and higher affinity·Animal-free production . . . .
Background References
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Images
ELISA of specificity for different species of IgG-Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH was used as the primary antibody. An HRP conjugated Anti-His Rabbit IgG(HA710110) as the secondary antibody.
A titer ELISA of mouse IgG3-Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH A titer ELISA of mouse IgG3. The plate was coated with different amounts of mouse IgG3. 1:10000 dilution of Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH was used as the primary antibody. An HRP conjugated Anti-His Rabbit IgG(HA710110) as the secondary antibody.
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