Biotin Conjugated Mouse IgG2b(Fcγ Fragment specific), AlpHcAbs® Goat antibody(Biotin) Alpaca pacous Monoclonal Antibody
Catalog# HA710134
Biotin Conjugated Mouse IgG2b(Fcγ Fragment specific), AlpHcAbs® Goat antibody(Biotin) Alpaca pacous Monoclonal Antibody
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WB
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ELISA
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Biotin
Overview
Product Name
Biotin Conjugated Mouse IgG2b(Fcγ Fragment specific), AlpHcAbs® Goat antibody(Biotin) Alpaca pacous Monoclonal Antibody
Antibody Type
Alpaca Nanobody
Immunogen
Recombinant Fc region of mouse IgG2b
Product Specificity
Mouse IgG2b (Fcγ fragment specific)
Validated Applications
WB, ELISA
Conjugation
Biotin
Product Features
Concentration
1mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C
Storage Buffer
10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Isotype
VHH domain of alpaca IgG2b/2c fused to goat IgG Fc
Purification Method
Recombinant Expression and Affinity purified
Application Dilution
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ELISA
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1:10000-1:50000
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WB
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1:10000-1:50000
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Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Target
Function
Anti-Mouse IgG2b(Fcγ Fragment specific), AlpHcAbs® Goat antibody(Biotin) is designed for detecting mouse IgG2b Fcγ fragment specifically. Anti-Mouse IgG2b(Fcγ Fragment specific), AlpHcAbs® Goat antibody(Biotin) is based on monoclonal, recombinant, goat IgG Fc fused single domain antibody to mouse IgG2b Fcγ fragment coupled to Biotin, and Anti-Mouse IgG2b(Fcγ Fragment specific), AlpHcAbs® Goat antibody(Biotin) reacts with the Fc fragment of mouse IgG2b selectively, no reactivity with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins., High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production . . . .
Background References
暂无
Images
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ELISA of specificity for different species of IgG-Anti-Mouse IgG2b(Fcγ Fragment specific), AlpHcAbs® Goat antibody(Biotin)
ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Mouse IgG2b(Fcγ Fragment specific), AlpHcAbs® Goat antibody(Biotin) was used as the primary antibody. An HRP conjugated streptavidin as the secondary antibody. -
A titer ELISA of mouse IgG2b-Anti-Mouse IgG2b(Fcγ Fragment specific), AlpHcAbs® Goat antibody(Biotin)
A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. 1:10000 dilution of Anti-Mouse IgG2b(Fcγ Fragment specific), AlpHcAbs® Goat antibody(Biotin) was used as the primary antibody. An HRP conjugated streptavidin as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"