VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Purification Method
Recombinant Expression and Affinity purified
Application Dilution
ELISA
1:5000-1:20000
WB
1:5000-1:20000
IP
1-2ug/sample
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Target
Function
Anti-Human kappa, AlpHcAbs® Goat antibody(Biotin) is designed for detecting human IgG kappa light chain specifically. Anti-Human kappa, AlpHcAbs® Goat antibody(Biotin) is based on monoclonal, recombinant, goat IgG Fc fused single domain antibody to human IgG kappa light chain coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Human kappa, AlpHcAbs® Goat antibody(Biotin) reacts with human IgG kappa light chain selectively, no reactivity with rabbit, mouse, rat, goat IgG. High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production . . . .
Background References
暂无
Images
Advantages of using an Alpaca Recombinant Secondary 1. Smaller size Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies. 2. Easier to use Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use. 3. Easier production When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies. 4. More consistent Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift. 5. Higher Affinity Nanobodies benefit from consistently high binding affinity values.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"