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V5 tag, AlpHcAbs® Rabbit Alpaca Monoclonal Antibody

Usd: 300

Specification

Bulk Order Quote | Customization Request

Catalog# HA710017

V5 tag, AlpHcAbs® Rabbit Alpaca Monoclonal Antibody

Application

  • WB

  • ICC/IF

  • ELISA

  • IP

  • Flow Cyt

Reactivity

  • Species independent

Conjugation

  • unconjugated

Overview

Product Name

V5 tag, AlpHcAbs® Rabbit Alpaca Monoclonal Antibody

Antibody Type

Alpaca Nanobody

Immunogen

V5 tag fusion protein

Product Specificity

V5 tag sequence(GKPIPNPLLGLDST)

Species Reactivity

Species independent

Validated Applications

WB, ICC/IF, ELISA, IP, Flow Cyt

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at –20 ℃, (Avoid freeze / thaw cycles), Stable for 12 months at -20℃

Storage Buffer

10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol

Isotype

VHH domain of alpaca IgG2b/2c fused to Rabbit Fc(mutation)

Purification Method

Recombinant Expression and Affinity purified

Application Dilution

  • WB

  • 1:10,000-1:50,000

  • ELISA

  • 1:10,000-1:50,000

  • ICC/IF

  • 1:200-1:1000

  • IP

  • 1-2ug/sample

  • Flow Cyt

  • 1µg for 106 cells

  • Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Target

Function

Anti-V5 tag, AlpHcAbs® Rabbit antibody is designed for detecting V5 tag fusion protein specifically. Anti-V5 tag, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc. Based on western blot and ELISA, Anti-V5 tag, AlpHcAbs® Rabbit antibody reacts with the V5 tag sequence(GKPIPNPLLGLDST) selectively, no reactivity with other proteins. High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production.

Background References

暂无

Images

  • Advantages of using an Alpaca Recombinant Secondary<br />1. Smaller size<br />Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies.<br />2. Easier to use<br />Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use. <br />3. Easier production<br />When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies. <br />4. More consistent<br />Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift.<br />5. Higher Affinity<br />Nanobodies benefit from consistently high binding affinity values.

    Advantages of using an Alpaca Recombinant Secondary
    1. Smaller size
    Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies.
    2. Easier to use
    Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use.
    3. Easier production
    When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies.
    4. More consistent
    Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift.
    5. Higher Affinity
    Nanobodies benefit from consistently high binding affinity values.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"