iFluor™ 647 Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody (HA1123)
Catalog# HA1123
Goat Polyclonal to Goat anti-rabbit IgG
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IF-Cell
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IF-Tissue
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IHC-Fr
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Rabbit
Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 3% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (ET1610-39) at 1/400 dilution in 3% BSA overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 647, HA1123) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Specifications
Product Type
Goat polyclonal secondary
Product Name
iFluor™ 647 Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody (HA1123)
Immunogen
Rabbit igg (h+l).
Specificity
To minimize cross-reactivity, the ifluor™ 594 conjugated goat anti-rabbit igg (h+l) antibodies have been highly cross-adsorbed against human igg and mouse igg.
Host
Goat
Conjugation
Ifluor™ 647
Clonality
Polyclonal
APPLICATION DILUTION
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IF-Cell
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1:500-1:1,000
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IF-Tissue
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1:500-1:1,000
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IHC-Fr
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1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Isotype
IgG
TARGET
PROTEIN NAME
Goat anti-rabbit IgG
FUNCTION
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. iFluor™ 647 dyes have fluorescence excitation and emission maxima of ~590 nm and ~610 nm respectively. iFluor™ 647 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative. iFluor™ 647 is much easier to be conjugated with RPE with much higher conjugation yield, and the resulted RPE-iFluor™ 647 tandem has better FRET efficiency. iFluor™ 647 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.
CITATIONS
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Mei Hongxiang,et al
Sema3A secreted by sensory nerve induces bone formation under mechanical loads
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IF
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Mouse
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Citation
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Parthenolide alleviates microglia‐mediated neuroinflammation via MAPK/TRIM31/NLRP3 signaling to ameliorate cognitive disorder
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IF
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Human
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Mouse
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Citation
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Igf1 Regulates Fibrocartilage Stem Cells, Cartilage Growth, and Homeostasis in the Temporomandibular Joint of Mice
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IF
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Human
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Mouse
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Citation
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Li Qianqian,et al
SAA1 regulated by S1P/S1PR1 promotes the progression of ESCC via β-catenin activation
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IF
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Hunan
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Citation
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Bi, R., Yin, Q., Li, H., Yang, X., Wang, Y., Li, Q., Fang, H., Li, P., Lyu, P., Fan, Y., Ying, B., & Zhu, S.
A single-cell transcriptional atlas reveals resident progenitor cell niche functions in TMJ disc development and injury
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IF
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Mouse
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Citation
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