Skip to content

Grp75 Mouse Monoclonal Antibody [A6-A5-B1]

Usd: 350

Specification

Bulk Order Quote | Customization Request

Catalog# M1603-1

Grp75 Mouse Monoclonal Antibody [A6-A5-B1]

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Grp75 Mouse Monoclonal Antibody [A6-A5-B1]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within Human Grp75 aa 630-679 / 679.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, FC

Molecular Weight

Predicted band size: 74 kDa

Positive Control

HeLa cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate, HeLa, NIH/3T3, HepG2.

Conjugation

unconjugated

Clone Number

A6-A5-B1

RRID

AB_3073159

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgM

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:100

  • FC

  • 1:50-1:100

Target

Function

The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. These proteins serve a variety of roles: they act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. All members of the family, except HSP 70, are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. Both HSP 70 and HSC 70 are involved in the chaperoning of nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity

Background References

1. Sato J et al. Family with Sequence Similarity 5, Member C (FAM5C) Increases Leukocyte Adhesion Molecules in Vascular Endothelial Cells: Implication in Vascular Inflammation. PLoS One 9:e107236 (2014).

2. Matin RN et al. p63 is an alternative p53 repressor in melanoma that confers chemoresistance and a poor prognosis. J Exp Med 210:581-603 (2013)

Sequence Similarity

Belongs to the heat shock protein 70 family.

Subcellular Location

Mitochondrion, Nucleus

UNIPROT

SwissProt: P38646 Human

SwissProt: P38647 Mouse

SwissProt: P48721 Rat

Synonyms

75 kDa glucose regulated protein antibody

75 kDa glucose-regulated protein antibody

CSA antibody

Glucose Regulated Protein antibody

Grp 75 antibody

GRP-75 antibody

GRP75 antibody

GRP75_HUMAN antibody

Heat shock 70 kDa protein 9 antibody

Heat shock 70kD protein 9 antibody

Expand

Images

  • Western blot analysis of Grp75 on different lysates with Mouse anti-Grp75 antibody (<a href="/products/M1603-1" style="font-weight: bold;text-decoration: underline;">M1603-1</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: K-562 cell lysate (20 µg/Lane)<br />Lane 3: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 4: PC-12 cell lysate (20 µg/Lane)<br />Lane 5: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 6: Mouse kidney tissue lysate (40 µg/Lane)<br />Lane 7: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 74 kDa<br />Observed band size: 74 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/M1603-1" style="font-weight: bold;text-decoration: underline;">M1603-1</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Grp75 on different lysates with Mouse anti-Grp75 antibody (M1603-1) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: K-562 cell lysate (20 µg/Lane)
    Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 4: PC-12 cell lysate (20 µg/Lane)
    Lane 5: Mouse liver tissue lysate (40 µg/Lane)
    Lane 6: Mouse kidney tissue lysate (40 µg/Lane)
    Lane 7: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 74 kDa
    Observed band size: 74 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1603-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HeLa cells labeling Grp75 with Mouse anti-Grp75 antibody (<a href="/products/M1603-1" style="font-weight: bold;text-decoration: underline;">M1603-1</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Grp75 antibody (<a href="/products/M1603-1" style="font-weight: bold;text-decoration: underline;">M1603-1</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling Grp75 with Mouse anti-Grp75 antibody (M1603-1) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Grp75 antibody (M1603-1) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of NIH/3T3 cells labeling Grp75 with Mouse anti-Grp75 antibody (<a href="/products/M1603-1" style="font-weight: bold;text-decoration: underline;">M1603-1</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Grp75 antibody (<a href="/products/M1603-1" style="font-weight: bold;text-decoration: underline;">M1603-1</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of NIH/3T3 cells labeling Grp75 with Mouse anti-Grp75 antibody (M1603-1) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Grp75 antibody (M1603-1) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

Grp75 Recombinant Mouse Monoclonal Antibody [6-A9-R]

Application: WB,IF-Cell,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Grp75 Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Grp75 Mouse Monoclonal Antibody [6-A9]

Application: WB

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Grp75 Recombinant Mouse Monoclonal Antibody [6-A9-R] - BSA and Azide free

Application: WB,IF-Cell,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Grp75 Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated