Golgin subfamily A member 5 Mouse Monoclonal Antibody [11-D7]
Catalog# M1004-6
Golgin subfamily A member 5 Mouse Monoclonal Antibody [11-D7]
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WB
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Human
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unconjugated
Overview
Product Name
Golgin subfamily A member 5 Mouse Monoclonal Antibody [11-D7]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human Golgin subfamily A member 5 aa 371-626 / 731.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 83 kDa
Positive Control
Hela cell lysate, K562 cell lysate, gastric cancer tissue.
Conjugation
unconjugated
Clone Number
11-D7
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2a
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-2000
Target
Function
Golgin subfamily A member 5(GOLGA5) is a golgi apparatus membrane protein, highly expressed in seminiferous tubules and Leydig cells in testis. It is highly phosphorylated during mitosis. GOLGA5 is involved in maintaining Golgi structure and intra-Golgi retrograde transport. It stimulates the formation of Golgi stacks and ribbons. Defects in GOLGA5 are a cause of thyroid papillary carcinoma (TPC).
Background References
1. "The coiled-coil membrane protein golgin-84 is a novel rab effector required for Golgi ribbon formation."Diao A., Rahman D., Pappin D.J.C., Lucocq J., Lowe M.J. Cell Biol. 160:201-212(2003)
2. "Golgin tethers define subpopulations of COPI vesicles."Malsam J., Satoh A., Pelletier L., Warren G.Science 307:1095-1098(2005)
Tissue Specificity
Ubiquitous. Highly expressed in seminiferous tubules and Leydig cells in testis, and detected at much lower levels in the other tissues tested. Expression is very low or not detectable in spermatozoa.
Post-translational Modification
Highly phosphorylated during mitosis. Phosphorylation is barely detectable during interphase.
Subcellular Location
Golgi apparatus membrane
UNIPROT
Synonyms
Cell proliferation inducing gene 31 antibody
Cell proliferation-inducing gene 31 protein antibody
GOGA5_HUMAN antibody
GOLGA 5 antibody
GOLGA5 antibody
Golgi autoantigen golgin subfamily a 5 antibody
Golgi integral membrane protein 5 antibody
Golgin 84 antibody
Golgin A5 antibody
Golgin subfamily A member 5 antibody
ExpandImages
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Sample:
Lane 1: K562 cell lysates
Lane 2: HepG2 cell lysates
Lane 3: A431 cell lysates
Primary: Anti-GOLGA5 (M1004-6) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 71 kD
Observed band size: 85 kD
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"