GPR132 Rabbit Polyclonal Antibody
Catalog# R1308-8
GPR132 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
GPR132 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human GPR132 aa 1-50 / 380.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 42 kDa
Positive Control
HeLa cell lysate, 293T cell lysate, Human brain tissue lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate, A172.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
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IF-Cell
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1:100
Target
Function
G protein coupled receptor 132, also termed G2A, is classified as a member of the proton sensing G protein coupled receptor (GPR) subfamily. Like other members of this subfamily, i.e. GPR4, OGR1 (GPR68), and TDAG8 (GPR65), G2A is a G protein coupled receptor that resides in the cell surface membrane, senses changes in extracellular pH, and can alter cellular function as a consequence of these changes. Subsequently, G2A was suggested to be a receptor for lysophosphatidylcholine (LPC). However, the roles of G2A as a pH-sensor or LPC receptor are disputed. Rather, current studies suggest that it is a receptor for certain metabolites of the polyunsaturated fatty acid, linoleic acid. G2A was initially defined as one of the gene products whose production was stimulated in mouse pre-B lymphocytes (see Immunoglobulin heavy chain) by transfecting the cells with the human oncogene (i.e., cancer causing) BCR-ABL or by treating the cells with DNA damaging agents; its expression in these cells blocked their progression through the cell cycle specifically at the G2-M DNA damage checkpoint. A report working with human neutrophils proposed that G2A was a receptor for a phospholipid, lysophosphatidylcholine (LPC), and a Sphingomyelin, sphingosylphosphorylcholine.
Background References
1. "The lysophospholipid receptor G2A activates a specific combination of G proteins and promotes apoptosis." Lin P., Ye R.D. J. Biol. Chem. 278:14379-14386(2003)
2. "A DNA damage and stress inducible G protein-coupled receptor blocks cells in G2/M." Weng Z., Fluckiger A.-C., Nisitani S., Wahl M.I., Le L.Q., Hunter C.A., Fernal A.A., Le Beau M.M., Witte O.N. Proc. Natl. Acad. Sci. U.S.A. 95:12334-12339(1998)
Sequence Similarity
Belongs to the G-protein coupled receptor 1 family.
Tissue Specificity
Highly expressed in macrophages and hematopoietic tissues rich in lymphocytes, like spleen and thymus. Weakly expressed in heart and lung. In atherosclerotic plaques, expression is observed around the lipid core and at the shoulder region.
Subcellular Location
Cell membrane.
Synonyms
G Protein Coupled Receptor G2A antibody
G2 accumulation protein antibody
G2A antibody
GP132_HUMAN antibody
GPR132 antibody
Probable G-protein coupled receptor 132 antibody
Images
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Western blot analysis of GPR132 on different lysates with Rabbit anti-GPR132 antibody (R1308-8) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: Human brain tissue lysate (40 µg/Lane)
Lane 4: Mouse skeletal muscle tissue lysate (40 µg/Lane)
Lane 5: Rat skeletal muscle tissue lysate (40 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 45 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-8) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunofluorescent staining of A172 cells using anti- GPR132 rabbit polyclonal antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"