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Fibrinogen alpha chain Recombinant Rabbit Monoclonal Antibody [JE37-07]

Usd: 385

Specification

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Catalog# HA722860

Fibrinogen alpha chain Recombinant Rabbit Monoclonal Antibody [JE37-07]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Fibrinogen alpha chain Recombinant Rabbit Monoclonal Antibody [JE37-07]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Fibrinogen alpha chain aa 761-810 / 866.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 95 kDa

Positive Control

Human plasma lysate, Mouse plasma lysate, Rat plasma lysate, human placenta tissue, mouse placenta tissue, rat placenta tissue, rat liver tissue.

Conjugation

unconjugated

Clone Number

JE37-07

Reactivity Data

WB IHC-P
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Fibrinogen alpha chain is a protein that in humans is encoded by the FGA gene. The protein encoded by this gene is the alpha component of fibrinogen, a blood-borne glycoprotein composed of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin, which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia, and renal amyloidosis. Alternative splicing results in two isoforms that vary in the carboxy-terminus.

Background References

1. Moazzeni A et al. Fibrinogen Aα gene genotyping in patients with inherited afibrinogenemia deficiency; a novel mutation in Iranian afibrinogenemia patients.Blood Coagul Fibrinolysis. 2023 Dec.

2. Çörtük M et al. Fibrinogen-Aα THR312ALA Polymorphism is Associated to Chronic Thromboembolic Pulmonary Hypertension in Turkey. Anatol J Cardiol. 2023 Mar.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P02671 Human

SwissProt: E9PV24 Mouse

SwissProt: P06399 Rat

Synonyms

Ac1873 antibody

Fba5e antibody

FGA antibody

Fib antibody

Fib2 antibody

FIBA_HUMAN antibody

Fibrinogen alpha chain antibody

Fibrinogen, A alpha polypeptide antibody

Fibrinogen--alpha polypeptide chain antibody

Fibrinopeptide A antibody

Images

  • Western blot analysis of Fibrinogen alpha chain on different lysates with Rabbit anti-Fibrinogen alpha chain antibody (<a href="/products/HA722860" style="font-weight: bold;text-decoration: underline;">HA722860</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human plasma lysate (30 µg/Lane)<br />Lane 2: Mouse plasma lysate (30 µg/Lane)<br />Lane 3: Rat plasma lysate (30 µg/Lane)<br /><br />Predicted band size: 95 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722860" style="font-weight: bold;text-decoration: underline;">HA722860</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Fibrinogen alpha chain on different lysates with Rabbit anti-Fibrinogen alpha chain antibody (HA722860) at 1/1,000 dilution.

    Lane 1: Human plasma lysate (30 µg/Lane)
    Lane 2: Mouse plasma lysate (30 µg/Lane)
    Lane 3: Rat plasma lysate (30 µg/Lane)

    Predicted band size: 95 kDa
    Observed band size: 100 kDa

    Exposure time: 59 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722860) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Fibrinogen alpha chain antibody (<a href="/products/HA722860" style="font-weight: bold;text-decoration: underline;">HA722860</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722860" style="font-weight: bold;text-decoration: underline;">HA722860</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Fibrinogen alpha chain antibody (HA722860) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722860) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-Fibrinogen alpha chain antibody (<a href="/products/HA722860" style="font-weight: bold;text-decoration: underline;">HA722860</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722860" style="font-weight: bold;text-decoration: underline;">HA722860</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-Fibrinogen alpha chain antibody (HA722860) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722860) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat placenta tissue with Rabbit anti-Fibrinogen alpha chain antibody (<a href="/products/HA722860" style="font-weight: bold;text-decoration: underline;">HA722860</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722860" style="font-weight: bold;text-decoration: underline;">HA722860</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat placenta tissue with Rabbit anti-Fibrinogen alpha chain antibody (HA722860) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722860) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Fibrinogen alpha chain antibody (<a href="/products/HA722860" style="font-weight: bold;text-decoration: underline;">HA722860</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722860" style="font-weight: bold;text-decoration: underline;">HA722860</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Fibrinogen alpha chain antibody (HA722860) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722860) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation