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Ferritin Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1601-9

Ferritin Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Ferritin Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human Ferritin aa 2-165.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 21 kDa

Positive Control

Rat spleen tissue lysate, human liver tissue, human liver cancer tissue, human kidney tissue, MCF-7, HepG2.

Conjugation

unconjugated

RRID

AB_3073407

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P FC IF-Cell
human
Tested Tested
 
Not Recommended
Not Recommended
mouse
 
Tested Tested
 
Not Recommended
rat
 
Tested Tested
 
Not Recommended

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:1,000-1:5,000

Target

Function

Mammalian ferritins consist of 24 subunits made up of two types of polypeptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts. The gene encoding human ferritin heavy chain maps to chromosome 11q13 and the human ferritin light chain gene maps to chromosome 19q13.3-q13.4.

Background References

1. Rychtarcikova Z et al. Tumor-initiating cells of breast and prostate origin show alterations in the expression of genes related to iron metabolism. Oncotarget 8:6376-6398 (2017).

2. Al-Eisawi Z et al. Novel Mechanism of Cytotoxicity for the Selective Selenosemicarbazone, 2-Acetylpyridine 4,4-Dimethyl-3-selenosemicarbazone (Ap44mSe): Lysosomal Membrane Permeabilization. J Med Chem 59:294-312 (2016).

Sequence Similarity

Belongs to the ferritin family.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P02792 Human

SwissProt: P02794 Human

SwissProt: P09528 Mouse

SwissProt: P29391 Mouse

SwissProt: P02793 Rat

SwissProt: P19132 Rat

Synonyms

Cell proliferation-inducing gene 15 protein antibody

Ferritin H subunit antibody

Ferritin heavy chain antibody

Ferritin heavy polypeptide 1 antibody

Ferritin L subunit antibody

Ferritin light polypeptide antibody

Ferritin, heavy polypeptide antibody

FRIH_HUMAN antibody

FTH antibody

FTH1 antibody

Expand

Images

  • Western blot analysis of Ferritin on rat spleen tissue lysates with Rabbit anti-Ferritin antibody (<a href="/products/R1601-9" style="font-weight: bold;text-decoration: underline;">R1601-9</a>) at 1:1000 dilution.<br /><br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 74 seconds; ECL: K1802<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/R1601-9" style="font-weight: bold;text-decoration: underline;">R1601-9</a>, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 21 kDa<br />Observed band size: 21 kDa

    Western blot analysis of Ferritin on rat spleen tissue lysates with Rabbit anti-Ferritin antibody (R1601-9) at 1:1000 dilution.


    Lysates/proteins at 10 µg/Lane.
    Exposure time: 74 seconds; ECL: K1802


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: R1601-9, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • Western blot analysis of Ferritin on different lysates with Rabbit anti-Ferritin antibody (<a href="/products/R1601-9" style="font-weight: bold;text-decoration: underline;">R1601-9</a>) at 1/1,000 dilution.<br /><br />Lane 1: FTL recombinant protein, 20ng/Lane<br />Lane 2: FTH1 recombinant protein, 20ng/Lane<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/R1601-9" style="font-weight: bold;text-decoration: underline;">R1601-9</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Ferritin on different lysates with Rabbit anti-Ferritin antibody (R1601-9) at 1/1,000 dilution.

    Lane 1: FTL recombinant protein, 20ng/Lane
    Lane 2: FTH1 recombinant protein, 20ng/Lane

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1601-9) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Ferritin antibody (<a href="/products/R1601-9" style="font-weight: bold;text-decoration: underline;">R1601-9</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/R1601-9" style="font-weight: bold;text-decoration: underline;">R1601-9</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Ferritin antibody (R1601-9) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1601-9) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Ferritin antibody (<a href="/products/R1601-9" style="font-weight: bold;text-decoration: underline;">R1601-9</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/R1601-9" style="font-weight: bold;text-decoration: underline;">R1601-9</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Ferritin antibody (R1601-9) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1601-9) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Ferritin antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Ferritin antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ferritin antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ferritin antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Ferritin antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Ferritin antibody. Counter stained with hematoxylin.

Citation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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