FCGRT Rabbit Polyclonal Antibody
Catalog# ER2001-44
FCGRT Rabbit Polyclonal Antibody
-
WB
-
Human
-
unconjugated
Overview
Product Name
FCGRT Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human FCGRT aa 20-70.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 40 kDa
Positive Control
Human brain tissue lysate, HepG2 cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
Target
Function
Cell surface receptor that transfers passive humoral immunity from the mother to the newborn. Binds to the Fc region of monomeric immunoglobulin gamma and mediates its selective uptake from milk . IgG in the milk is bound at the apical surface of the intestinal epithelium. The resultant FcRn-IgG complexes are transcytosed across the intestinal epithelium and IgG is released from FcRn into blood or tissue fluids. Throughout life, contributes to effective humoral immunity by recycling IgG and extending its half-life in the circulation. Mechanistically, monomeric IgG binding to FcRn in acidic endosomes of endothelial and hematopoietic cells recycles IgG to the cell surface where it is released into the circulation . In addition of IgG, regulates homeostasis of the other most abundant circulating protein albumin/ALB .
Background References
1. Story C.M. et. al. A major histocompatibility complex class I-like Fc receptor cloned from human placenta: possible role in transfer of immunoglobulin G from mother to fetus. J. Exp. Med. 180:2377-2381(1994).
Subcellular Location
Cell membrane, Endosome, Membrane.
UNIPROT
Synonyms
Alpha chain antibody
Fc fragment of IgG, receptor transporter, alpha antibody
FCGRN_HUMAN antibody
FCGRT antibody
FcRn alpha chain antibody
FcRn antibody
FCRN, alpha chain antibody
IgG Fc fragment receptor transporter alpha chain antibody
IgG Gc receptor antibody
IgG receptor FcRn large subunit p51 antibody
ExpandImages
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Western blot analysis of FCGRT on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human brain tissue lysate
Lane 2: HepG2 cell lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"