F13A1 Mouse Monoclonal Antibody [16D1]
Catalog# EM1901-39
F13A1 Mouse Monoclonal Antibody [16D1]
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WB
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IF-Cell
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
F13A1 Mouse Monoclonal Antibody [16D1]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human F13A1 aa 259-463 / 732.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P, FC
Molecular Weight
Predicted band size: 83 kDa
Positive Control
Human placenta tissue lysates, human placenta tissue, MCF-7, MG-63, A549.
Conjugation
unconjugated
Clone Number
16D1
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2a
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:100
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IHC-P
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1:200-1:1,000
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FC
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1:50-1:100
Target
Function
Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (prothrombin, and factors V, VIII, IX and X), which are involved in a blood coagulation cascade leading to the formation of insoluble fibrin clots and the promotion of platelet aggregation. Coagulation factor VII (serum prothrombin conversion accelerator, proconvertin, F7, Factor VII) is a 406 amino acid, vitamin K-dependent, single chain serine protease that is synthesized in the liver and circulates as an inactive precursor. Factor IXa, factor Xa, factor XIIa, or thrombin mediated proteolytic cleavage of Factor VII at Arg152-Ile153 generates Factor VIIa, an active serine protease composed of a catalytic heavy chain disulfide linked to a light chain, containing two EGF-like domains. Coagulation factor XIII is a terminal effector in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of two A subunits and two B subunits. The A subunits have catalytic function, and the noncatalytic B subunits may serve as plasma carrier molecules.
Background References
1. Thomas A. et. al. Coagulation factor XIIIA subunit missense mutations affect structure and function at the various steps of factor XIII action. Hum. Mutat. 37:1030-1041(2016).
Sequence Similarity
Belongs to the transglutaminase superfamily. Transglutaminase family.
Post-translational Modification
The activation peptide is released by thrombin.
Subcellular Location
Secreted, cytoplasm.
UNIPROT
Synonyms
bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
Coagulation factor XIII A chain antibody
Coagulation factor XIII A1 polypeptide antibody
Coagulation factor XIII A1 subunit antibody
Coagulation factor XIII, A polypeptide antibody
Coagulation factor XIIIa antibody
F13A antibody
F13A_HUMAN antibody
F13a1 antibody
Factor XIIIA antibody
ExpandImages
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Western blot analysis of F13A1 on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-39, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
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ICC staining of F13A1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-39, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of F13A1 in MG-63 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-39, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-F13A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-39, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of F13A1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-39, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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