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Estrogen Receptor alpha Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1803-83

Estrogen Receptor alpha Rabbit Polyclonal Antibody

Application

  • WB

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Estrogen Receptor alpha Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within Human Estrogen Receptor alpha aa 1-120.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, FC

Molecular Weight

66/54 kDa

Positive Control

293T cell lysates, MCF-7.

Conjugation

unconjugated

RRID

AB_3069277

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

Target

Function

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades.

Background References

1. Stein B et al. Repression of the interleukin-6 promoter by estrogen receptor is mediated by NF-kappa B and C/EBP beta. Mol Cell Biol 15:4971-4979 (1995).

2. Porter W et al. Functional synergy between the transcription factor Sp1 and the estrogen receptor. Mol Endocrinol 11:1569-1580 (1997).

Sequence Similarity

Belongs to the nuclear hormone receptor family. NR3 subfamily.

Tissue Specificity

Widely expressed. Isoform 3 is not expressed in the pituitary gland.

Post-translational Modification

Phosphorylated by cyclin A/CDK2 and CK1. Phosphorylation probably enhances transcriptional activity. Self-association induces phosphorylation. Dephosphorylation at Ser-118 by PPP5C inhibits its transactivation activity. Phosphorylated by LMTK3 in vitro.; Glycosylated; contains N-acetylglucosamine, probably O-linked.; Ubiquitinated; regulated by LATS1 via DCAF1 it leads to ESR1 proteasomal degradation. Deubiquitinated by OTUB1.; Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization. Demethylated by JMJD6 at Arg-260.; Palmitoylated (isoform 3). Not biotinylated (isoform 3).; Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation, but not for signaling mediated by the nuclear hormone receptor.

Subcellular Location

Cytoplasm. Nucleus. Cell membrane. Golgi apparatus.

UNIPROT

SwissProt: P03372 Human

SwissProt: P19785 Mouse

SwissProt: P06211 Rat

Synonyms

DKFZp686N23123 antibody

ER alpha antibody

ER antibody

ER-alpha antibody

Era antibody

ESR antibody

ESR1 antibody

ESR1_HUMAN antibody

ESRA antibody

Estradiol receptor antibody

Expand

Images

  • Western blot analysis of Estrogen Receptor alpha on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Estrogen Receptor alpha on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of Estrogen Receptor alpha was done on MCF-7 cells. The cells were fixed, permeabilized and stained with Estrogen Receptor alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for 1 hour, the cells was stained with a Alexa Fluor&trade; 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.

    Flow cytometric analysis of Estrogen Receptor alpha was done on MCF-7 cells. The cells were fixed, permeabilized and stained with Estrogen Receptor alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for 1 hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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