Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody [SY88-19]
Catalog# ET1605-17
Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody [SY88-19]
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WB
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody [SY88-19]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein with Human Cytokeratin 5 aa 1-590.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P
Target Molecular Weight
62 kDa
Positive Control
A431 cell lysates, human tonsil tissue, human lung cancer tissue, human breast cancer tissue.
Conjugation
unconjugated
Clone Number
SY88-19
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:50-1:200
Target
Function
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratin 5 is expressed in normal basal cells. Mutations of the cytokeratin 5 gene (KRT5) have been shown to result in the autosomal dominant disorder epidermolysis bullosa (EB).
Background References
1. Khanom R et al. Keratin 17 Is Induced in Oral Cancer and Facilitates Tumor Growth. PLoS One 11:e0161163 (2016).
2. Dorà NJ et al. Analysis of compound heterozygotes reveals that the mouse floxed Pax6 (tm1Ued) allele produces abnormal eye phenotypes. Transgenic Res 25:679-92 (2016).
Sequence Similarity
Belongs to the intermediate filament family.
Tissue Specificity
Expressed in corneal epithelium (at protein level).
Subcellular Location
Cytoplasm,nucleus.
Synonyms
58 kDa cytokeratin antibody
CK-5 antibody
CK5 antibody
Cytokeratin-5 antibody
Cytokeratin5 antibody
DDD antibody
DDD1 antibody
EBS2 antibody
epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody
K2C5_HUMAN antibody
ExpandImages
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Western blot analysis of Cytokeratin 5 on A431 cell lysates with Rabbit anti-Cytokeratin 5 antibody (ET1605-17) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 62 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-17) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human brest cancer tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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