Estrogen Receptor alpha Rabbit Polyclonal Antibody
Catalog# 0803-1
Estrogen Receptor alpha Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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Human
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unconjugated
Overview
Product Name
Estrogen Receptor alpha Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within C terminal human estrogen receptor.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 66 kDa
Positive Control
MCF-7, human breast tissue, SK-Br-3.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:100
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IF-Cell
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1:50
Target
Function
Estrogen receptor alpha (ERα), also known as NR3A1 (nuclear receptor subfamily 3, group A, member 1), is one of two main types of estrogen receptor, a nuclear receptor that is activated by the sex hormone estrogen. In humans, ERα is encoded by the gene ESR1 (EStrogen Receptor 1). The estrogen receptor (ESR) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The ESR1 protein localizes to the nucleus where it may forms a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
Background References
1. Karas RH, Patterson BL, Mendelsohn ME. Human vascular smooth muscle cells contain functional estrogen receptor［J］. Circulation, 1994, 89(5): 1943.
2. Grohe C, Kahlert K, Van EM, et al. Effects of moediprilat on oestrogen-stimulated cardiac fibroblast growth［J］. Br J Pharmacol, 1997,121(7):1350.
Sequence Similarity
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Tissue Specificity
Widely expressed. Isoform 3 is not expressed in the pituitary gland.
Post-translational Modification
Phosphorylated by cyclin A/CDK2 and CK1. Phosphorylation probably enhances transcriptional activity. Self-association induces phosphorylation. Dephosphorylation at Ser-118 by PPP5C inhibits its transactivation activity. Phosphorylated by LMTK3 in vitro.; Glycosylated; contains N-acetylglucosamine, probably O-linked.; Ubiquitinated; regulated by LATS1 via DCAF1 it leads to ESR1 proteasomal degradation. Deubiquitinated by OTUB1.; Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization. Demethylated by JMJD6 at Arg-260.; Palmitoylated (isoform 3). Not biotinylated (isoform 3).; Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation, but not for signaling mediated by the nuclear hormone receptor.
Subcellular Location
Nucleus, Cytoplasm, Cell membrane.
UNIPROT
Synonyms
DKFZp686N23123 antibody
ER alpha antibody
ER antibody
ER-alpha antibody
Era antibody
ESR antibody
ESR1 antibody
ESR1_HUMAN antibody
ESRA antibody
Estradiol receptor antibody
ExpandImages
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Western blot analysis on MCF-7 using anti-ESR1 polyclonal antibody
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Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Estrogen Receptor alpha antibody (0803-1) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0803-1) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of SK-Br-3 cells labeling Estrogen Receptor alpha with Rabbit anti-Estrogen Receptor alpha antibody (0803-1) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Estrogen Receptor alpha antibody (0803-1) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"