B7-H4(VTCN1) Rabbit Polyclonal Antibody
Catalog# ER1802-56
B7-H4(VTCN1) Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Rat
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unconjugated
Overview
Product Name
B7-H4(VTCN1) Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human B7-H4 aa 25-259.
Species Reactivity
Human, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 31 kDa
Positive Control
SKBR3 cell and human colon tissue lysates, MCF-7, rat epididymid tissue, human breast tissue, human breast tissue, human kidney tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2000
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IF-Cell
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1:1,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory proteins. One such protein, B7-H4 (B7-homolog 4, also designated VTCN1), belongs to the B7 Immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immunoinhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells supresses tumor-associated immunity. Current research suggests that, similar to Mucin 16 (CA125), B7-H4 may be a useful biomarker for the early detection of ovarian cancer.
Background References
1. Salceda S et al. The immunomodulatory protein B7-H4 is overexpressed in breast and ovarian cancers and promotes epithelial cell transformation. Exp Cell Res 306:128-141 (2005).
2. Kryczek I et al. B7-H4 expression identifies a novel suppressive macrophage population in human ovarian carcinoma. J Exp Med 203:871-881 (2006).
Sequence Similarity
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Tissue Specificity
Overexpressed in breast, ovarian, endometrial, renal cell (RCC) and non-small-cell lung cancers (NSCLC). Expressed on activated T- and B-cells, monocytes and dendritic cells, but not expressed in most normal tissues (at protein level). Widely expressed, including in kidney, liver, lung, ovary, placenta, spleen and testis.
Post-translational Modification
N-glycosylated.
Subcellular Location
Cell membrane.
Synonyms
B7 family member, H4 antibody
B7 H4 antibody
B7 homolog 4 antibody
B7 superfamily member 1 antibody
B7 superfamily, member 1 antibody
B7-H4 antibody
B7h.5 antibody
B7h4 antibody
B7S1 antibody
B7x antibody
ExpandImages
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Western blot analysis of B7-H4(VTCN1) on SKBR3 cell and human colon tissue lysates using anti-B7-H4(VTCN1) antibody at 1/500 dilution.
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ICC staining B7-H4(VTCN1) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-B7-H4(VTCN1) antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-B7-H4(VTCN1) antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-B7-H4(VTCN1) antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
B7H4 Recombinant Rabbit Monoclonal Antibody [PSH03-65]
Application: WB,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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