USP36 Rabbit Polyclonal Antibody
Catalog# ER1706-23
USP36 Rabbit Polyclonal Antibody
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IF-Cell
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IHC-P
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Human
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unconjugated
Overview
Product Name
USP36 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human USP36 aa 1,074-1,123 / 1,123.
Species Reactivity
Human
Validated Applications
IF-Cell, IHC-P
Target Molecular Weight
123 kDa
Positive Control
A549, Hela, LOVO, human liver cancer tissue, human spleen tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
Target
Function
Ubiquitin-specific protease 36 is an enzyme that in humans is encoded by the USP36 gene. This gene encodes a member of the ubiquitin-specific protease family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the nucleoli and deubuquitinate nucleophosmin and fibrillarin. May be required for maintaining multiple types of adult stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4).
Background References
1. Puente XS et al. Human and mouse proteases: a comparative genomic approach. Nat Rev Genet 4 (7): 544–558 (2003).
2. Endo A et al. Nucleolar structure and function are regulated by the deubiquitylating enzyme USP36". J Cell Sci 122: 678–86 (2009).
Sequence Similarity
Belongs to the peptidase C19 family.
Tissue Specificity
Broadly expressed.
Post-translational Modification
Polyubiquitinated by NEDD4L, no effect on USP36 protein levels. Both proteins interact with and regulate each other's ubiquitination levels.
Subcellular Location
Nucleus.
UNIPROT
Synonyms
Deubiquitinating enzyme 1 antibody
Deubiquitinating enzyme 36 antibody
DUB1 antibody
FLJ12851 antibody
KIAA1453 antibody
Ubiquitin carboxyl-terminal hydrolase 36 antibody
Ubiquitin specific peptidase 36 antibody
Ubiquitin specific processing protease 36 antibody
Ubiquitin thioesterase 36 antibody
Ubiquitin thiolesterase 36 antibody
ExpandImages
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ICC staining USP36 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining USP36 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining USP36 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-USP36 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-USP36 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"