Taq DNA Polymerase Mouse Monoclonal Antibody [3C3]

Function

Polymerase Chain Reaction (PCR), invented by Kary B. Mullis, at the Cetus Corporation, who was awarded the 1993 Nobel Prize for chemistry for PCR, is a technique to exponentially amplify in vitro a small quantity of a specific nucleotide sequence using a thermostable (Taq) DNA polymerase. Anti-Taq Antibody is an ideal tool for hot-start PCR with Taq DNA polymerase. The Anti-Taq Antibody binds to Taq DNA polymerase and arrests the activity of Taq DNA Polymerase, preventing non-specific and primer dimer amplification resulted from non-specific priming at ambient temperature for the duration of time prior to PCR thermal cycling. During the initial denaturing step in PCR thermal cycling, the Anti-Taq Antibody is denatured and the Taq DNA polymerase is then released, thus regaining its full DNA polymerase activity. The result indicates that anti-Taq DNA Polymerase antibody increases the specificity and sensitivity of the PCR.

Background References

1. Kellogg DE et al. TaqStart Antibody: "hot start" PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase. Biotechniques. 16(6):1134-7 (1994).

2. Sharkey DJ et al. Antibodies as thermolabile switches: high temperature triggering for the polymerase chain reaction. Biotechnology (N Y). 12(5):506-9 (1994).