Skip to content

Alpha 1 Acid Glycoprotein Mouse Monoclonal Antibody [A2-B10]

Usd:

Bulk Order Quote | Customization Request

Catalog# EM1510-41

Alpha 1 Acid Glycoprotein Mouse Monoclonal Antibody [A2-B10]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Alpha 1 Acid Glycoprotein Mouse Monoclonal Antibody [A2-B10]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human ORM1 aa 1-201 / 201.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

Predicted band size: 24 kDa

Positive Control

Rat liver tissue, human liver tissue, mouse liver tissue, human esophagus tissue, HepG2.

Conjugation

unconjugated

Clone Number

A2-B10

RRID

AB_3068741

Product Features

Form

Liquid

Concentration

2 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2a

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:1,000

  • FC

  • 1:50-1:100

Target

Function

This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be involved in aspects of immunosuppression. Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction.

Background References

1. Fitos I et al. Selective binding of imatinib to the genetic variants of human alpha1-acid glycoprotein. Biochim Biophys Acta 1760:1704-1712(2006).

2. Zsila F et al. The drug binding site of human alpha1-acid glycoprotein: insight from induced circular dichroism and electronic absorption spectra. Biochim Biophys Acta 1770:797-809(2007).

Sequence Similarity

Belongs to the calycin superfamily. Lipocalin family.

Tissue Specificity

Expressed by the liver and secreted in plasma.

Post-translational Modification

N-glycosylated. N-glycan heterogeneity at Asn-33: Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor).

Subcellular Location

Secreted.

UNIPROT

SwissProt: P02763 Human

SwissProt: Q60590 Mouse

SwissProt: P02764 Rat

Synonyms

A1AG1_HUMAN antibody

AGP 1 antibody

AGP A antibody

AGP antibody

AGP1 antibody

Alpha 1 acid glycoprotein antibody

Alpha-1-acid glycoprotein 1 antibody

alpha-1-AGP antibody

Epididymis secretory sperm binding protein Li 153w antibody

glycoprotein, alpha-1-acid, of serum antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti- Alpha 1 Acid Glycoprotein antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti- Alpha 1 Acid Glycoprotein antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Alpha 1 Acid Glycoprotein antibody (EM1510-41) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (EM1510-41) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Alpha 1 Acid Glycoprotein antibody (EM1510-41) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1510-41) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-Alpha 1 Acid Glycoprotein antibody (EM1510-41) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (EM1510-41) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-Alpha 1 Acid Glycoprotein antibody (EM1510-41) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1510-41) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti- Alpha 1 Acid Glycoprotein antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti- Alpha 1 Acid Glycoprotein antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Alternative Products