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Mouse CCL2/MCP-1 enzyme-linked immunoassay kit (EM0025)

Price: Regular price $499.00

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Catalog# EM0025

Assay type Sandwich ELISA

Standard curve range 6.86~5000 pg/ml

Sensitivity 1.78 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.00740.00680.0071
6.860.01150.01140.01150.0044
20.580.01500.01660.01580.0087
61.730.03180.03260.03220.0251
185.190.08930.09860.09400.0869
555.560.29210.30090.29650.2894
1666.671.12801.16201.14501.1379
5000.003.59703.57473.58593.5788

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)122.5532.21655.9135.6526.31598.4
Standard Deviation9.123.568.18.424.160.7
Coefficient of Variation(%)7.44.44.16.24.63.8

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum3029.88-82.7110051.74

Sensitivity

1.78 pg/ml (10 μl)

Standard curve range

6.86~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse CCL2 into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 86% to 119% with an overall mean recovery of 108%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse CCL2/MCP-1 enzyme-linked immunoassay kit (EM0025)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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