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Mouse/Rat IGF-I/IGF-1 enzyme-linked immunoassay kit(EM0024)

Price: Regular price $499.00

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Catalog# EM0024

Assay type Sandwich ELISA

Standard curve range 6.86~5000 pg/ml

Sensitivity 1.76 pg/ml (50 μl)；3.43 pg/ml (10 μl)

Reacts with

  • Mouse

  • Rat

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.06470.06350.0641
6.860.13850.13030.13440.0703
20.580.27350.27240.27300.2089
61.730.56180.52790.54490.4808
185.191.05500.95491.00500.9409
555.561.62701.53901.58301.5189
1666.672.23902.01302.12602.0619
5000.002.63202.68202.65702.5930

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)119.3509.01409.9109.9510.11498.1
Standard Deviation8.239.3109.45.427.0100.4
Coefficient of Variation(%)6.97.77.84.95.36.7

Sample Values

Sample MatrixSample EvaluatedRange (ng/ml)Detectable (%) Mean of Detectable (ng/ml)
Serum304.93-460.32100135.06

Sensitivity

1.76 pg/ml (50 μl)；3.43 pg/ml (10 μl)

Standard curve range

6.86~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of Mouse/Rat IGF-I/IGF-1 into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 94% to 105% with an overall mean recovery of 101%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse/Rat IGF-I/IGF-1 enzyme-linked immunoassay kit(EM0024)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

15 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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