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Mouse IL-21 enzyme-linked immunoassay kit(EM0020)

Price: Regular price $499.00

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Catalog# EM0020

Assay type Sandwich ELISA

Standard curve range 78.13~5000 pg/ml

Sensitivity 3.22 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.02820.02700.0276
78.130.06900.07790.07350.0459
156.250.11520.12190.11860.0910
312.500.22420.23560.22990.2023
625.000.40890.43310.42100.3934
1250.000.84300.82960.83630.8087
2500.001.62601.70101.66351.6359
5000.003.28503.29603.29053.2629

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)122.1592.61834.3106.1524.21569.8
Standard Deviation8.039.388.36.121.173.4
Coefficient of Variation(%)6.66.64.85.84.04.7

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-5.8966.72.50

Sensitivity

3.22 pg/ml (10 μl)

Standard curve range

78.13~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse IL-21 into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 80% to 96% with an overall mean recovery of 89%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse IL-21 enzyme-linked immunoassay kit(EM0020)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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