Mouse CXCL1/KC enzyme-linked immunoassay kit(EM0017)
Catalog# EM0017
Assay type Sandwich ELISA
Standard curve range 13.72~10000 pg/ml
Sensitivity 2.11 pg/ml (10 μl)
-
Mouse
PERFORMANCE CHARACTERISTICS
Product detail
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0104 | 0.0085 | 0.0095 | |
| 13.72 | 0.0192 | 0.0193 | 0.0193 | 0.0098 |
| 41.15 | 0.0391 | 0.0402 | 0.0397 | 0.0302 |
| 123.46 | 0.0841 | 0.0835 | 0.0838 | 0.0743 |
| 370.37 | 0.2060 | 0.2181 | 0.2121 | 0.2026 |
| 1111.11 | 0.6431 | 0.6263 | 0.6347 | 0.6252 |
| 3333.33 | 1.6330 | 1.8620 | 1.7475 | 1.7380 |
| 10000.00 | 4.0881 | 4.1300 | 4.1091 | 4.0996 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 217.4 | 917.4 | 3162.0 | 158.9 | 706.9 | 2846.8 |
| Standard Deviation | 14.6 | 51.4 | 187.2 | 9.2 | 29.9 | 172.5 |
| Coefficient of Variation(%) | 6.7 | 5.6 | 5.9 | 5.8 | 4.2 | 6.1 |
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 43.94-268.81 | 100 | 66.43 |
Sensitivity
2.11 pg/ml (10 μl)
Standard curve range
13.72~10000 pg/ml
Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CXCL1/KC into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 88% to 109% with an overall mean recovery of 96%.
Specifications
Product Type
ELISA Kit
Product Name
Mouse CXCL1/KC enzyme-linked immunoassay kit(EM0017)
Detection method
Colorimetric
Sample type
Serum
Assay type
Sandwich ELISA
Category
Fully Ready-to-Use
PROPERTIES
Storage instructions
Store at +4℃
Kits Components
Coated Plate
96 well
Standard
9 vial
Detect Antibody
6 ml/bottle
Streptavidin-HRP
12 ml/bottle
Assay Buffer
12 ml/bottle
Stop Solution
12 ml/bottle
Wash Buffer
50 ml/bottle
Sealer Film
6 pieces
CITATIONS
-
Fuxiang Li,et al
SIRT7 remodels the cytoskeleton via RAC1 to enhance host resistance to Mycobacterium tuberculosis
-
Elisa
-
Mouse;Human
-
Citation
-
