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Mouse IL-12/IL-23 p40 enzyme-linked immunoassay kit(EM0012)

Price: Regular price $499.00

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Catalog# EM0012

Assay type Sandwich ELISA

Standard curve range 156.25~10000 pg/ml

Sensitivity 1.65 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.02270.02300.0229
156.250.18020.17250.17640.1535
312.500.32420.30880.31650.2937
625.000.58630.58050.58340.5606
1250.001.12201.15001.13601.1132
2500.002.24301.99002.11652.0937
5000.003.54903.48903.51903.4962
10000.004.40604.51614.46114.4382

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)254.31384.43649.7229.41118.23402.2
Standard Deviation6.743.2130.413.263.8131.2
Coefficient of Variation(%)2.63.13.65.85.73.9

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30662.49-1451.181001102.18

Sensitivity

1.65 pg/ml (10 μl)

Standard curve range

156.25~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse IL-12/IL-23 p40 into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 80% to 119% with an overall mean recovery of 97%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse IL-12/IL-23 p40 enzyme-linked immunoassay kit(EM0012)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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