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Mouse IL-17A enzyme-linked immunoassay kit(EM0008)

Price: Regular price $499.00

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Catalog# EM0008

Assay type Sandwich ELISA

Standard curve range 4.12~3000 pg/ml

Sensitivity 0.25 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01850.01900.0188
4.120.04010.04100.04060.0218
12.350.07800.07930.07870.0599
37.040.17410.17380.17400.1552
111.110.45440.45130.45290.4341
333.331.07931.10091.09011.0714
1000.002.47512.48112.47812.4594
3000.004.27124.36984.32054.3018

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)40.2254.7867.443235.5947.6
Standard Deviation1.98.739.61.77.644.7
Coefficient of Variation(%)4.83.44.63.93.24.7

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-21.67336.01

Sensitivity

0.25 pg/ml (10 μl)

Standard curve range

4.12~3000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse IL-17A into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 76% to 108% with an overall mean recovery of 95%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse IL-17A enzyme-linked immunoassay kit(EM0008)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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