Human CKMB enzyme-linked immunoassay kit

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Catalog# EH0072

Human CKMB enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

0.04~30 ng/ml

Conjugation

unconjugated

Overview

Product Name

Human CKMB enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.005 pg/ml (50 μl)；0.07 ng/ml (10 μl)

ng/ml	O.D.		Average	Corrected
0.00	0.0144	0.0158	0.0151
0.04	0.0332	0.0309	0.0321	0.0170
0.12	0.0716	0.0726	0.0721	0.0570
0.37	0.1778	0.1780	0.1779	0.1628
1.11	0.4794	0.4464	0.4629	0.4478
3.33	1.3260	1.3850	1.3555	1.3404
10.00	3.2180	3.2230	3.2205	3.2054
30.00	4.5063	4.4600	4.4832	4.4681

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (ng/ml)	0.7	3.8	13.1	0.6	3.2	11.0
Standard Deviation	0.1	0.2	1.0	0.1	0.1	0.3
Coefficient of Variation (%)	5.6	4.6	7.3	3.6	3.9	3.1

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human CKMB into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 79% to 119% with an overall mean recovery of 103%.

Sample Value

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	n.d.-0.06	40	0.02

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CKMB in this assay. No medical histories were available for the donors.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt:P06732

SwissProt:P12277

Alias

A2MR

Alpha 2 macroglobulin receptor

alpha 2MR

Alpha-2-macroglobulin receptor

APOER

Apolipoprotein E receptor

APR

CD 91

CD91

CD91 antigen

Expand

Function

The creatine kinase molecule is a dimer composed of a brain-type subunit (B) and a muscle-type subunit (M). There are usually three isozymes in normal human tissues, which are CK-BB (CK1), CK-MB (CK2) and CK-MM (CK3) in the order of electrophoresis. CK-BB is mainly found in smooth muscle and brain tissue, and almost none in serum; CK-MB mainly exists in myocardial tissue, accounting for 0~4% of CK in serum; CK-MM is mainly present in skeletal muscle, accounting for 96%~100% of CK in serum. CK-MB has its own subtypes, including MB2 (tissue form) and MB1 (transformed form). After the release of CK-MB2 from the myocardium, the carboxy-terminus is hydrolyzed by plasma carboxypeptidase N, and a lysine residue is removed to be converted to the serum-modified isoform CK-MB1. CK and CK-MB are one of the sensitive indicators of myocardial tissue injury, especially CK-MB is more specific. The increase in CK during myocardial injury is mainly due to the increase in CK-MB, which is manifested by the simultaneous increase of both.

Human CKMB enzyme-linked immunoassay kit

Usd: Special Discount

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Alias

Function

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