Human ICAM-1 / CD54 enzyme-linked immunoassay kit
Catalog# EH0064
Human ICAM-1 / CD54 enzyme-linked immunoassay kit
-
ELISA
-
Human
-
41.15~30000 pg/ml
-
unconjugated
Overview
Product Name
Human ICAM-1 / CD54 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.49 pg/ml (50 μl)；6.36 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0073 | 0.0073 | 0.0073 | |
| 41.15 | 0.0092 | 0.0095 | 0.0094 | 0.0021 |
| 123.46 | 0.0167 | 0.0149 | 0.0158 | 0.0085 |
| 370.37 | 0.0389 | 0.0368 | 0.0379 | 0.0306 |
| 1111.11 | 0.1437 | 0.1413 | 0.1425 | 0.1352 |
| 3333.33 | 0.6950 | 0.6774 | 0.6862 | 0.6789 |
| 10000.00 | 2.1350 | 2.0700 | 2.1025 | 2.0952 |
| 30000.00 | 3.7320 | 3.6030 | 3.6675 | 3.6602 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 750.8 | 2955.8 | 8132.1 | 699.4 | 3236.1 | 8913.5 |
| Standard Deviation | 38.6 | 163.5 | 460.8 | 29.1 | 188.7 | 535.4 |
| Coefficient of Variation (%) | 5.1 | 5.5 | 5.7 | 4.2 | 5.8 | 6.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human ICAM-1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 97% to 103% with an overall mean recovery of 99%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 111.33-174.86 | 100 | 140.01 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of ICAM-1 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Antigen identified by monoclonal
BB2
BB 2
BB2
CD 54
CD_antigen=CD54
CD54
Cell surface glycoprotein P3.58
Human rhinovirus receptor
ICAM 1
Expand