Human ICAM-1 / CD54 enzyme-linked immunoassay kit
Catalog# EH0064
Human ICAM-1 / CD54 enzyme-linked immunoassay kit
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ELISA
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Human
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13.72~10000 pg/ml
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unconjugated
Overview
Product Name
Human ICAM-1 / CD54 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
4.30 pg/ml(50 μl)；34.71 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0128 | 0.0150 | 0.0139 | |
| 13.72 | 0.0217 | 0.0241 | 0.0229 | 0.0090 |
| 41.15 | 0.0372 | 0.0347 | 0.0360 | 0.0221 |
| 123.46 | 0.0720 | 0.0770 | 0.0745 | 0.0606 |
| 370.37 | 0.1703 | 0.1777 | 0.1740 | 0.1601 |
| 1111.11 | 0.4687 | 0.4474 | 0.4581 | 0.4442 |
| 3333.33 | 1.2790 | 1.2190 | 1.2490 | 1.2351 |
| 10000.00 | 2.8090 | 2.9300 | 2.8695 | 2.8556 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 234.1 | 1082.2 | 3166.5 | 213.4 | 1189.1 | 3504.5 |
| Standard Deviation | 19.8 | 43.9 | 70.1 | 17.3 | 46.1 | 211.0 |
| Coefficient of Variation (%) | 8.4 | 4.1 | 2.2 | 8.1 | 3.9 | 6.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human ICAM-1/CD54 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 97% to 120% with an overall mean recovery of 118%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 46.75-110.67 | 100.0 | 88.85 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of ICAM-1 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Antigen identified by monoclonal
BB2
BB 2
BB2
CD 54
CD_antigen=CD54
CD54
Cell surface glycoprotein P3.58
Human rhinovirus receptor
ICAM 1
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