Human TGF-α enzyme-linked immunoassay kit
Catalog# EH0059
Human TGF-α enzyme-linked immunoassay kit
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ELISA
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Human
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4.12~3000 pg/ml
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unconjugated
Overview
Product Name
Human TGF-α enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.91 pg/ml (50 μl)；3.89 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0422 | 0.0382 | 0.0402 | |
| 4.12 | 0.0471 | 0.0527 | 0.0499 | 0.0439 |
| 12.35 | 0.0726 | 0.0712 | 0.0719 | 0.0659 |
| 37.04 | 0.1285 | 0.1285 | 0.1285 | 0.1225 |
| 111.11 | 0.3091 | 0.3135 | 0.3113 | 0.3053 |
| 333.33 | 0.7566 | 0.7792 | 0.7679 | 0.7619 |
| 1000.00 | 1.7560 | 1.6690 | 1.7125 | 1.7065 |
| 3000.00 | 3.1350 | 3.1530 | 3.1440 | 3.1380 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 59.6 | 296.2 | 1122.0 | 58.2 | 320.6 | 1180.8 |
| Standard Deviation | 2.7 | 20.8 | 74.4 | 2.3 | 16.3 | 40.8 |
| Coefficient of Variation (%) | 4.5 | 7.0 | 6.6 | 4.0 | 5.1 | 3.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human TGF-α into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 104% to 126% with an overall mean recovery of 114%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-25.28 | 50 | 9.57 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of TGF-α in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
EGF like TGF
EGF-like TGF
ETGF
TFGA
TGF A
TGF type 1
TGF-alpha
Tgfa
TGFA_HUMAN
Transforming growth factor alpha
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