Human/Monkey/Canine Osteopontin（OPN） enzyme-linked immunoassay kit
Catalog# EH0057
Human/Monkey/Canine Osteopontin（OPN） enzyme-linked immunoassay kit
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ELISA
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Human
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0.31~20 ng/ml
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unconjugated
Overview
Product Name
Human/Monkey/Canine Osteopontin（OPN） enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.04 ng/ml (50 μl)；0.12 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0246 | 0.0244 | 0.0245 | |
| 0.31 | 0.0543 | 0.0502 | 0.0523 | 0.0278 |
| 0.63 | 0.0937 | 0.0866 | 0.0902 | 0.0657 |
| 1.25 | 0.2017 | 0.1903 | 0.1960 | 0.1715 |
| 2.50 | 0.5132 | 0.5027 | 0.5080 | 0.4835 |
| 5.00 | 1.0570 | 0.9935 | 1.0253 | 1.0008 |
| 10.00 | 1.9270 | 1.8780 | 1.9025 | 1.8780 |
| 20.00 | 3.0580 | 2.9620 | 3.0100 | 2.9855 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 0.42 | 2.09 | 6.22 | 0.51 | 2.10 | 6.03 |
| Standard Deviation | 0.02 | 0.09 | 0.48 | 0.02 | 0.10 | 0.37 |
| Coefficient of Variation (%) | 4.9 | 4.5 | 7.7 | 4.3 | 4.7 | 6.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human OPN into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 117% with an overall mean recovery of 108%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Human Serum | 30 | 1.10-6.74 | 100 | 3.97 |
| Monkey Serum | 2 | 6.28-6.57 | 100 | 6.43 |
| Canine Serum | 5 | 1.22-1.43 | 100 | 1.33 |
Serum/Plasma – Thirty samples from apparently healthy volunteers, two samples from healthy monkeys and five samples from healthy canines were evaluated for the presence of human OPN in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
BNSP
Bone sialoprotein 1
BSP I
BSPI
Early T lymphocyte activation 1
ETA 1
ETA1
MGC110940
Nephropontin
OPN
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