Human IFN-gamma enzyme-linked immunoassay kit

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Catalog# EH0039

Human IFN-gamma enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

1.37~1000 pg/ml

Conjugation

unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Human IFN-gamma enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.36 pg/ml (50 μl)；0.85 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0117	0.0121	0.0119
1.37	0.0250	0.0282	0.0266	0.0147
4.12	0.0462	0.0489	0.0476	0.0357
12.35	0.1248	0.1240	0.1244	0.1125
37.04	0.3399	0.3393	0.3396	0.3277
111.11	0.9201	0.8954	0.9078	0.8959
333.33	2.0160	1.9910	2.0035	1.9916
1000.00	3.6940	3.6730	3.6835	3.6716

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	17.2	98.2	273.3	18.9	91.8	240.1
Standard Deviation	0.5	3	6.8	0.8	2.7	5.2
Coefficient of Variation (%)	2.8	3.2	2.5	4.2	3	2.1

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human IFN gamma into health human serum sample. The un-spiked serum was used as blank in this experiment.

The recovery ranged from 89% to 113% with an overall mean recovery of 105%

Sample Value

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	n.d.-446.28	92	93.56

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IFN-gamma in this assay. No medical histories were available for the donors.

n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

75min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Application in Publications

ELISA

See 1 publications below

Application in Publications

Human

See 1 publications below

Target

UNIPROT

SwissProt: P01579 Human

Alias

IFG

IFI

IFN gamma

IFN, immune

IFN-gamma

IFNG

IFNG_HUMAN

Immune interferon

Interferon gamma

Function

Interferon-gamma (IFN-gamma), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59%‑64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37%‑43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Citation

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A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment
- Journal: Bioactive Materials
- DOI: 10.1016/j.bioactmat.2024.11.005
- IF: 18
- Application: ELISA
- Reactivity: Human
- Publish date: 2024 Dec

Human IFN-gamma enzyme-linked immunoassay kit

Usd: Special Discount

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Application in Publications

Application in Publications

Target

UNIPROT

Alias

Function

Citation

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