Human Asprosin enzyme-linked immunoassay kit
Catalog# EH0032
Human Asprosin enzyme-linked immunoassay kit
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ELISA
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Human
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4.69~300 ng/ml
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unconjugated
Overview
Product Name
Human Asprosin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.78 ng/ml (50 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0407 | 0.0468 | 0.0438 | |
| 4.69 | 0.1037 | 0.1032 | 0.1035 | 0.0597 |
| 9.38 | 0.1406 | 0.1638 | 0.1522 | 0.1085 |
| 18.75 | 0.2698 | 0.2895 | 0.2797 | 0.2359 |
| 37.50 | 0.5256 | 0.5766 | 0.5511 | 0.5074 |
| 75.00 | 1.0950 | 1.1600 | 1.1275 | 1.0838 |
| 150.00 | 2.3230 | 2.0280 | 2.1755 | 2.1318 |
| 300.00 | 3.6020 | 3.3780 | 3.4900 | 3.4463 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 6.4 | 29.6 | 112.8 | 5.9 | 31.2 | 118.1 |
| Standard Deviation | 0.3 | 1.2 | 5.9 | 0.3 | 1.3 | 5.4 |
| Coefficient of Variation (%) | 4.0 | 4.0 | 5.2 | 5.1 | 4.2 | 4.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 3.35-128.74 | 100 | 34.32 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Asprosin in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
350 kDa glycoprotein component extracellular microfibril
ACMICD
FBN 1
FBN
FBN1
FBN1_HUMAN
Fibrillin 15
Fibrillin-1
Fibrillin1
Fibrillin15
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