Human IL-15 enzyme-linked immunoassay kit
Catalog# EH0031
Human IL-15 enzyme-linked immunoassay kit
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ELISA
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Human
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15.63~1000 pg/ml
Overview
Product Name
Human IL-15 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.91 pg/ml (50 μl) ； 4.20 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0569 | 0.0510 | 0.0540 | |
| 15.63 | 0.0986 | 0.0931 | 0.0959 | 0.0419 |
| 31.25 | 0.1385 | 0.1383 | 0.1384 | 0.0845 |
| 62.50 | 0.2296 | 0.2298 | 0.2297 | 0.1758 |
| 125.00 | 0.4239 | 0.4303 | 0.4271 | 0.3732 |
| 250.00 | 0.8343 | 0.8272 | 0.8308 | 0.7768 |
| 500.00 | 1.5410 | 1.4950 | 1.5180 | 1.4641 |
| 1000.00 | 2.8480 | 2.7570 | 2.8025 | 2.7486 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 20.0 | 97.8 | 305.8 | 25.0 | 120.8 | 332.4 |
| Standard Deviation | 1.0 | 7.6 | 14.0 | 1.8 | 9.4 | 17.0 |
| Coefficient of Variation (%) | 4.2 | 4.6 | 4.9 | 4.8 | 4.7 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-15 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 120% with an overall mean recovery of 109%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| serum | 30 | n.d.-14.63 | 6.7 | 14.63 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-15 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
L15、 IL-15、IL-15MGC9721、interleukin 15、interleukin-15