Human HGF enzyme-linked immunoassay kit
Catalog# EH0019
Human HGF enzyme-linked immunoassay kit
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ELISA
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Human
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10.97~8000 pg/ml
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unconjugated
Overview
Product Name
Human HGF enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
4.07 pg/ml (50 μl)；6.01 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0284 | 0.0277 | 0.0281 | |
| 10.97 | 0.0360 | 0.0382 | 0.0371 | 0.0091 |
| 32.92 | 0.0521 | 0.0501 | 0.0511 | 0.0231 |
| 98.77 | 0.1109 | 0.0958 | 0.1034 | 0.0753 |
| 296.30 | 0.2883 | 0.2774 | 0.2829 | 0.2548 |
| 888.89 | 0.8003 | 0.8033 | 0.8018 | 0.7738 |
| 2666.67 | 2.3398 | 2.3380 | 2.3389 | 2.3109 |
| 8000.00 | 3.9661 | 4.0435 | 4.0048 | 3.9768 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 190.1 | 1075.3 | 3244.1 | 214.2 | 1168.4 | 3780.4 |
| Standard Deviation | 12.4 | 73.9 | 219.8 | 15.2 | 69.0 | 144.5 |
| Coefficient of Variation (%) | 6.5 | 6.9 | 6.8 | 7.1 | 5.9 | 3.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human HGF into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 88% to 121% with an overall mean recovery of 108%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 360.14-1072.52 | 100 | 609.53 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of HGF in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
DFNB39
F TCF
Fibroblast derived tumor cytotoxic factor
Hepatocyte growth factor (hepapoietin A
scatter factor)
Hepatocyte growth factor
Hepatocyte growth factor beta chain
Hepatocyte growth factor precursor
Hepatopoietin A
Hepatopoietin-A
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