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Human IL-2 enzyme-linked immunoassay kit (EH0013)

Price: Regular price $499.00

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Catalog# EH0013

Assay type Sandwich ELISA

Standard curve range 6.86~5000 pg/ml

Sensitivity 1.67 pg/ml (50 μl) ；3.08 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01120.01110.0112
6.860.02120.01730.01930.0085
20.580.03010.02820.02920.0175
61.730.06710.06230.06470.0530
185.190.21420.19830.20630.1955
555.560.87210.77230.82220.8105
1666.672.49522.40732.45132.4401
5000.004.29724.31734.30734.2961

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)92.1407.51540.9102.1502.11990.9
Standard Deviation4.723.486.46.029.1119.09
Coefficient of Variation(%)5.15.75.65.95.86.0

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.00

Sensitivity

1.67 pg/ml (50 μl) ；3.08 pg/ml (10 μl)

Standard curve range

6.86~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-2 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 85% to 115% with an overall mean recovery of 103%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-2 enzyme-linked immunoassay kit (EH0013)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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