Human IL-2 enzyme-linked immunoassay kit
Catalog# EH0013
Human IL-2 enzyme-linked immunoassay kit
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ELISA
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Human
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78.13~5000 pg/ml
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unconjugated
Overview
Product Name
Human IL-2 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.77 pg/ml (50 μl) ； 3.97 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0093 | 0.0085 | 0.0089 | |
| 78.13 | 0.0663 | 0.0726 | 0.0695 | 0.0606 |
| 156.25 | 0.1299 | 0.1416 | 0.1358 | 0.1269 |
| 312.50 | 0.2600 | 0.2822 | 0.2711 | 0.2622 |
| 625.00 | 0.5334 | 0.5230 | 0.5282 | 0.5193 |
| 1250.00 | 1.1330 | 1.1310 | 1.1320 | 1.1231 |
| 2500.00 | 1.8960 | 1.8960 | 1.8960 | 1.8871 |
| 5000.00 | 3.3500 | 3.3630 | 3.3565 | 3.3476 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 61.2 | 515.4 | 1587.3 | 86.0 | 481.5 | 1469.9 |
| Standard Deviation | 4.2 | 17.0 | 54.5 | 4.8 | 18.1 | 99.9 |
| Coefficient of Variation (%) | 6.9 | 3.3 | 3.4 | 5.6 | 3.8 | 6.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 102% with an overall mean recovery of 86%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d. | 0 | 0 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-2 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 1 publications below |
Application in Publications
| Human | See 1 publications below |
Target
UNIPROT
Alias
Aldesleukin
IL 2
IL-2
IL2
IL2_HUMAN
Interleukin 2
Interleukin-2
interleukin2
Involved in regulation of T cell clonal expansion
Lymphokine
ExpandFunction
Citation
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A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment
Journal: Bioactive Materials
DOI: 10.1016/j.bioactmat.2024.11.005
IF: 18
Application: ELISA
Reactivity: Human
Publish date: 2024 Dec