Human IL-10 enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

96T

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Catalog# EH0009

Human IL-10 enzyme-linked immunoassay kit

Application

sELISA

Reactivity

Human

Standard curve range

15.63~1000 pg/ml

Conjugation

unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Human IL-10 enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.35 pg/ml（50 μl）；0.61 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0268	0.0259	0.0264
15.63	0.0433	0.0397	0.0415	0.0152
31.25	0.0795	0.0831	0.0813	0.0550
62.50	0.1977	0.1791	0.1884	0.1621
125.00	0.4669	0.4558	0.4614	0.4350
250.00	1.1354	1.2026	1.1690	1.1427
500.00	2.7050	2.7878	2.7464	2.7201
1000.00	3.5502	3.5608	3.5555	3.5292

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	16.87	106.92	324.44	17.85	99.67	293.71
Standard Deviation	1.07	4.78	11.70	1.34	7.33	20.34
Coefficient of Variation (%)	6.3	4.5	3.6	7.5	7.4	6.9

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-10 into health human serum sample. The un-spiked serum was used as blank in these experiments.

The recovery ranged from 94% to 109% with an overall mean recovery of 101%.

Sample Value

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	3.07-8.49	100	5.19

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-10 in this assay. No medical histories were available for the donors.

n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points

Incubation Count

Detectable Sample

Dosing Volume

10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Application in Publications

ELISA

See 7 publications below

Application in Publications

Human

See 7 publications below

Target

UNIPROT

SwissProt: P22301 Human

Alias

CSIF

Cytokine synthesis inhibitory factor

GVHDS

IL 10

IL-10

IL10

IL10_HUMAN

IL10A

Interleukin 10

Interleukin-10

Expand

Function

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. Mature mouse IL-10 shares 85% amino acid sequence identity with rat IL-10 and 70-77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer. IL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta. IL-10 R alpha is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells, while the 75 kDa transmembrane IL-10 R beta is widely expressed. The IL-10 dimer binds to two IL 10 R alpha chains, triggering recruitment of two IL-10 R beta chains. IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29. The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages. Its expression by immunosuppressive regulatory T cells (Treg) and regulatory B cells is important for Treg proliferation. Within a tumor microenvironment, however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells. IL-10 induces the intratumoral accumulation and activation of CD8+ T cells. IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. The levels of IL-10 are elevated in Sjogren's syndrome (saliva), primary CNS lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites). Its levels are decreased in the serum in patients with recurrent heart attacks or during preeclampsia and also in the seminal fluid of infertile men.

Citation

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miR-324-3p Drives Malignant Behaviors of Tumor Cells by Promoting M2 Macrophage Polarization in Laryngeal Squamous Cell Carcinoma
- Journal: Tohoku Journal Of Experimental Medicine
- DOI: 10.1620/tjem.2025.J083
- IF: 1.6
- Application: ELISA
- Reactivity: Human
- Publish date: 2026 Mar
The TPx Protein of Cysticercus cellulosae Promotes an IL-10–Producing Regulatory B Cell Phenotype in Human B Lymphoblastoid Cells via the cAMP/CREB Pathway
- Journal: Cells
- DOI: 10.3390/cells15161429
- IF: 6
- Application: ELISA
- Reactivity: Human
- Publish date: 2026 Aug
Framework nucleic acids as structural nanomedicine for alleviating multi-organ injury in sepsis
- Journal: Journal Of Nanobiotechnology
- DOI: 10.1186/s12951-026-05006-9
- IF: 15
- Application: ELISA
- Reactivity: Human
- Publish date: 2026 Aug
MIR4435–2HG promotes breast cancer evolution through miR-205-5p/UBE2N axis and exosome-mediated macrophage M2-like polarization
- Journal: Cellular Signalling
- DOI: 10.1016/j.cellsig.2025.112287
- IF: 3.7
- Application: ELISA
- Reactivity: Human
- Publish date: 2025 Nov
Multi-omics insights of immune cells in the risk and prognosis of idiopathic membranous nephropathy
- Journal: Communications Biology
- DOI: 10.1038/s42003-025-08642-3
- IF: 5.1
- Application: ELISA
- Reactivity: Human
- Publish date: 2025 Aug
Indoleamine 2,3-dioxygenase 1 alters the proportions of B cell subpopulations in the microenvironment of acute myeloid leukemia
- Journal: Molecular Biomedicine
- DOI: 10.1186/s43556-025-00262-x
- IF: 6.3
- Application: ELISA
- Reactivity: Human
- Publish date: 2025 Apr
A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment
- Journal: Bioactive Materials
- DOI: 10.1016/j.bioactmat.2024.11.005
- IF: 18
- Application: ELISA
- Reactivity: Human
- Publish date: 2024 Dec

Human IL-10 enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Application in Publications

Application in Publications

Target

UNIPROT

Alias

Function

Citation

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