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Human IL-4 enzyme-linked immunoassay kit (EH0007)

Price: Regular price $499.00

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Catalog# EH0007

Assay type Sandwich ELISA

Standard curve range 1.37~1000 pg/ml

Sensitivity 0.03 pg/ml (50 μl) ；0.28 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
00.00890.00850.0087
1.370.01630.01680.01660.0079
4.120.0310.03710.03410.0254
12.350.08550.09070.08810.0794
37.040.24980.25530.25260.2439
111.110.71430.73350.72390.7152
333.331.92491.92641.92571.917
10003.85913.77553.81733.8086

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)24.9124.6243.428.5126.1219.8
Standard Deviation1.35.58.21.966.4
Coefficient of Variation(%)3.44.55.16.54.72.9

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-0.49800.13

Sensitivity

0.03 pg/ml (50 μl) ；0.28 pg/ml (10 μl)

Standard curve range

1.37~1000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-4 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 88% to 105% with an overall mean recovery of 98%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-4 enzyme-linked immunoassay kit (EH0007)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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