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Human IL-1β enzyme-linked immunoassay kit (EH0006)

Price: Regular price $499.00

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Catalog# EH0006

Assay type Sandwich ELISA

Standard curve range 3.91~250 pg/ml

Sensitivity 0.18 pg/ml (10 μl)；0.09 pg/ml (50 μl)；

Reacts with

  • Human

Safety datasheet

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.02960.03070.0302
3.910.13300.12320.12810.0980
7.810.21320.21220.21270.1826
15.630.38200.37600.37900.3489
31.250.74060.69800.71930.6892
62.501.37101.28801.32951.2994
125.002.44902.30602.37752.3474
250.003.76203.62303.69253.6624

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)6.029.693.77.233.6104.4
Standard Deviation0.31.34.10.31.02.0
Coefficient of Variation(%)4.84.54.44.12.91.9

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum301.56-60.8710015.59

Sensitivity

0.18 pg/ml (10 μl)；0.09 pg/ml (50 μl)；

Standard curve range

3.91~250 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-1 beta into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 85% to 120% with an overall mean recovery of 95%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-1β enzyme-linked immunoassay kit (EH0006)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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