Human TNF-α enzyme-linked immunoassay kit (EH0002)
Catalog# EH0002
Assay type Sandwich ELISA
Standard curve range 1.37~1000 pg/ml
Sensitivity 0.21 pg/ml (50 μl) ；0.26 pg/ml (10 μl)
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Human
PERFORMANCE CHARACTERISTICS
Product detail
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0390 | 0.0402 | 0.0396 | |
| 1.37 | 0.0588 | 0.0628 | 0.0608 | 0.0212 |
| 4.12 | 0.0819 | 0.0885 | 0.0852 | 0.0456 |
| 12.35 | 0.1693 | 0.1712 | 0.1703 | 0.1307 |
| 37.04 | 0.3876 | 0.3956 | 0.3916 | 0.3520 |
| 111.11 | 0.9071 | 0.9969 | 0.9520 | 0.9124 |
| 333.33 | 2.4084 | 2.3694 | 2.3889 | 2.3493 |
| 1000.00 | 4.3651 | 4.1387 | 4.2519 | 4.2123 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 13 | 74.8 | 215.6 | 15.3 | 73.9 | 323.3 |
| Standard Deviation | 0.5 | 3.1 | 7.3 | 0.6 | 2.8 | 12.9 |
| Coefficient of Variation(%) | 3.7 | 4.1 | 3.4 | 4 | 3.8 | 4 |
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.63-3.85 | 100 | 2.08 |
Sensitivity
0.21 pg/ml (50 μl) ；0.26 pg/ml (10 μl)
Standard curve range
1.37~1000 pg/ml
Recovery
The spike recovery was evaluated by spiking 3 levels of human TNF alpha into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 95% to 105% with an overall mean recovery of 99%.
Specifications
Product Type
ELISA Kit
Product Name
Human TNF-α enzyme-linked immunoassay kit (EH0002)
Detection method
Colorimetric
Sample type
Serum
Assay type
Sandwich ELISA
Category
Fully Ready-to-Use
PROPERTIES
Storage instructions
Store at +4℃
Kits Components
Coated Plate
96 well
Standard
9 vial
Detect Antibody
6 ml/bottle
Streptavidin-HRP
12 ml/bottle
Assay Buffer
12 ml/bottle
Stop Solution
12 ml/bottle
Wash Buffer
50 ml/bottle
Sealer Film
6 pieces
CITATIONS
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Yinfeng Xu,et al
The cGAS-STING pathway activates transcription factor TFEB to stimulate lysosome biogenesis and pathogen clearance
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ELISA
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Human
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Citation
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