KIAA0100 Mouse Monoclonal Antibody [A0-F3]
Catalog# M1210-6
KIAA0100 Mouse Monoclonal Antibody [A0-F3]
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WB
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IF-Cell
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IHC-P
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Human
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unconjugated
Overview
Product Name
KIAA0100 Mouse Monoclonal Antibody [A0-F3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human KIAA0100 aa 1,947-2,235 / 2,235.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P
Target Molecular Weight
Predicted band size: 254 kDa
Positive Control
Hela, human breast cancer tissue, human kidney tissue, human pancreas tissue.
Conjugation
unconjugated
Clone Number
A0-F3
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:10,000
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IF-Cell
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1:100-1:500
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IHC-P
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1:2,000
Target
Function
Breast cancer-overexpressed gene 1 protein (KIAA0100) is a glucoprotein composed of 2,235 amino acids. KIAA0100 has four isforms (254kDa,250kDa,45kDa,25kDa).KIAA0100 is expressed in pancreas, placenta and up-regulated in breast carcinoma epithelial cells. This gene was initially characterized in human as having high expression levels in breast carcinomas and breast cancer cell lines. This gene also has increased expression in prostrate cancer cells relative to normal prostrate tissues. Expression of this gene is negatively regulated by direct binding of the microRNA miR-195 to its 3' UTR. miR-195 has been shown to modulate the invasiveness of prostrate cancer cells and xenograft metastases by downgrading expression of this gene. In mouse, the protein encoded by this gene was identified as an antigen on acute monocytic leukemia cells. In human, alternative splicing results in multiple transcript variants encoding distinct isoforms; some of these isoforms are predicted to contain an RNA pol II promoter FMP27 protein domain and a Golgi-body-localization APT1 domain.
Background References
1. "A quantitative atlas of mitotic phosphorylation."Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008)
2. "Serological identification of immunogenic antigens in acute monocytic leukemia."Chen G., Zhang W.G., Cao X.M., Li F.Y., Liu X.P., Yao L.B.Leuk. Res. 29:503-509(2005)
Sequence Similarity
Belongs to the SABRE family.
Tissue Specificity
Expressed in pancreas, placenta and up-regulated in breast carcinoma epithelial cells, ductal in situ carcinoma (DCIS), invasive breast carcinoma (IBC) and metastatic breast carcinoma cells (MET).
Subcellular Location
Cell membrane, Endoplasmic reticulum membrane, Mitochondrion membrane.
UNIPROT
Synonyms
Antigen MLAA-22 antibody
BCOX antibody
BCOX1 antibody
Breast cancer-overexpressed gene 1 protein antibody
CT101 antibody
DKFZp686M0843 antibody
K0100_HUMAN antibody
KIAA0100 antibody
MGC111488 antibody
MGC134981 antibody
ExpandImages
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Immunofluorescent staining of Hela cells using anti-KIAA0100 Mouse mAb (Cat. # M1210-6).
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-KIAA0100 antibody (M1210-6) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1210-6) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-KIAA0100 antibody (M1210-6) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1210-6) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-KIAA0100 antibody (M1210-6) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1210-6) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of KIAA0100 on different lysates with Mouse anti-KIAA0100 antibody (M1210-6) at 1/1,000 dilution.
Lane 1: MDA-MB-231(Human triple-negative breast cancer cells) cell lysate
Lane 2: SK-Br-3(Human breast cancer cells) cell lysate
Lane 3: MCF7 (Human breast cancer cell) cell lysate
Lane 4: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 5: A431 (human epidermal carcinoma cells) cell lysate
Lane 6: PC-3M(Human prostate cancer cells) cell lysate
Lane 7: LNCaP (Human prostate cancer cells) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 1 minutes 2 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: M1210-6, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 253.7 kDa
Observed band size: 40-50,254 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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