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BHMT Mouse Monoclonal Antibody [9E1]

Usd: 350

Specification

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Catalog# EM1701-64

BHMT Mouse Monoclonal Antibody [9E1]

Application

  • WB

  • IHC-P

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

BHMT Mouse Monoclonal Antibody [9E1]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein with Human BHMT aa 250-406 / 406.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Cell, FC

Molecular Weight

Predicted band size: 45 kDa

Positive Control

Human liver tissue lysate, human kidney tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, SiHa, HepG2.

Conjugation

unconjugated

Clone Number

9E1

RRID

AB_3068788

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:20,000

  • IF-Cell

  • 1:50

  • IHC-P

  • 1:2,000

  • FC

  • 1:50-1:100

Target

Function

Betaine-homocysteine methyltransferase (BHMT) is a zinc-dependent cytosolic protein that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT is highly expressed in rat liver, and its expression is regulated by dietary methionine and choline. In humans, S-adenosylmethionine (SAM) down-regulates BHMT expression by inducing NFκB, which acts as a repressor for the BHMT gene. Lowered BHMT levels can lead to ER (endoplasmic reticulum) stress. Mutations in the gene encoding for BHMT may lead to hyperhomocysteinemia, a medical condition characterized by abnormally large amounts of homocysteine in the blood which may be a risk factor for cardiovascular and cerebrovascular diseases.

Background References

1. Sunden S L F et al. Betaine-homocysteine methyltransferase expression in porcine and human tissues and chromosomal localization of the human gene. Arch Biochem Biophys 345:171-174 (1997).

2. Evans J C et al. Betaine-homocysteine methyltransferase: zinc in a distorted barrel. Structure 10:1159-1171 (2002).

Tissue Specificity

Found exclusively in liver and kidney.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q93088 Human

SwissProt: 329582 Mouse

SwissProt: O09171 Rat

Synonyms

Betaine homocysteine methyltransferase antibody

Betaine homocysteine S methyltransferase 1 antibody

Betaine homocysteine S methyltransferase antibody

Betaine--homocysteine S-methyltransferase 1 antibody

BHMT antibody

BHMT1 antibody

BHMT1_HUMAN antibody

EC 2.1.1.5 antibody

Epididymis secretory sperm binding protein Li 61p antibody

HEL S 61p antibody

Images

  • Western blot analysis of BHMT on different lysates with Mouse anti-BHMT antibody (<a href="/products/EM1701-64" style="font-weight: bold;text-decoration: underline;">EM1701-64</a>) at 1/2,000 dilution.<br /><br />Lane 1: Human liver tissue lysate<br />Lane 2: Human kidney tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 5 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1701-64" style="font-weight: bold;text-decoration: underline;">EM1701-64</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (<a href="/products/NBI02H" style="font-weight: bold;text-decoration: underline;">NBI02H</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of BHMT on different lysates with Mouse anti-BHMT antibody (EM1701-64) at 1/2,000 dilution.

    Lane 1: Human liver tissue lysate
    Lane 2: Human kidney tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

    Exposure time: 5 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-64) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-BHMT antibody (<a href="/products/EM1701-64" style="font-weight: bold;text-decoration: underline;">EM1701-64</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1701-64" style="font-weight: bold;text-decoration: underline;">EM1701-64</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-BHMT antibody (EM1701-64) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-64) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-BHMT antibody (<a href="/products/EM1701-64" style="font-weight: bold;text-decoration: underline;">EM1701-64</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1701-64" style="font-weight: bold;text-decoration: underline;">EM1701-64</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-BHMT antibody (EM1701-64) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-64) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-BHMT antibody (<a href="/products/EM1701-64" style="font-weight: bold;text-decoration: underline;">EM1701-64</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1701-64" style="font-weight: bold;text-decoration: underline;">EM1701-64</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-BHMT antibody (EM1701-64) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-64) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ICC staining BHMT (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining BHMT (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining BHMT (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining BHMT (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Alternative Products

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BHMT Recombinant Mouse Monoclonal Antibody [9E1-R]

Application: WB,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated