Alkaline Phosphatase(PLAP-1/PLAP-like) Rabbit Polyclonal Antibody
Catalog# 0806-4
Alkaline Phosphatase(PLAP-1/PLAP-like) Rabbit Polyclonal Antibody
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WB
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
Alkaline Phosphatase(PLAP-1/PLAP-like) Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human Alkaline Phosphatase(PLAP-1 / PLAP-like) aa 377-426 / 535.
Species Reactivity
Human
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
Predicted band size: 58 kDa
Positive Control
Hela cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:5000
Target
Function
Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Placental ALP is highly polymorphic, there are at least three common alleles. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Background References
1. Ezra E., Blacher R., Udenfriend S.; "Purification and partial sequencing of human placental alkaline phosphatase."; Biochem. Biophys. Res. Commun. 116:1076-1083(1983).
2. Garattini E., Hua J.-C., Pan Y.C.E., Udenfriend S.; "Human liver alkaline phosphatase, purification and partial sequencing: homology with the placental isozyme."; Arch. Biochem. Biophys. 245:331-337(1986).
Sequence Similarity
Belongs to the alkaline phosphatase family.
Tissue Specificity
Detected in placenta (at protein level).
Subcellular Location
Cell membrane, Lipid-anchor, GPI-anchor.
UNIPROT
Synonyms
Alkaline phosphatase antibody
Alkaline phosphatase placental antibody
Alkaline phosphatase placental type antibody
Alkaline phosphatase Regan isozyme antibody
ALP antibody
Alp1 antibody
ALPP antibody
FLJ61142 antibody
Germ-cell alkaline phosphatase antibody
nagao isozyme antibody
ExpandImages
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Western blot analysis of Alkaline Phosphatase(PLAP-1/PLAP-like) on different lysates with Rabbit anti-Alkaline Phosphatase(PLAP-1/PLAP-like) antibody (0806-4) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: SiHa (Human cervical cancersSquamous cell) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: 0806-4, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 58 kDa
Observed band size: 58 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Placental alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [JM22-53]
Application: WB,IF-Tissue,IHC-P
Reactivity: Human
Conjugate: unconjugated
