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ATBF1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER2001-49

ATBF1 Rabbit Polyclonal Antibody

Application

  • WB

  • FC

Reactivity

  • Human

Predicted reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

ATBF1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human ATBF1 aa 2100-2200.

Species Reactivity

Human (Predicted: Mouse, Rat)

Predicted species support after-sales service

Validated Applications

WB, FC

Target Molecular Weight

404 kDa

Positive Control

MCF-7 cell lysates, HepG2.

Conjugation

unconjugated

RRID

AB_3069526

Product Features

Form

Liquid

Concentration

1 mg/ml.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

Target

Function

Transcriptional regulator which can act as an activator or a repressor. Inhibits the enhancer element of the AFP gene by binding to its AT-rich core sequence. In concert with SMAD-dependent TGF-beta signaling can repress the transcription of AFP via its interaction with SMAD2/3 (PubMed:25105025). Regulates the circadian locomotor rhythms via transcriptional activation of neuropeptidergic genes which are essential for intercellular synchrony and rhythm amplitude in the suprachiasmatic nucleus (SCN) of the brain (By similarity). Regulator of myoblasts differentiation through the binding to the AT-rich sequence of MYF6 promoter and promoter repression . Down-regulates the MUC5AC promoter in gastric cancer . In association with RUNX3, upregulates CDKN1A promoter activity following TGF-beta stimulation . Inhibits estrogen receptor (ESR1) function by selectively competing with coactivator NCOA3 for binding to ESR1 in ESR1-positive breast cancer cells .

Background References

1. Berry F.B. et. al. Positive and negative regulation of myogenic differentiation of C2C12 cells by isoforms of the multiple homeodomain zinc finger transcription factor ATBF1. J. Biol. Chem. 276:25057-25065(2001).

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: Q15911 Human

Synonyms

Alpha fetoprotein enhancer binding protein antibody

AT binding transcription factor 1 antibody

AT motif binding factor antibody

ATBT antibody

ZFHX3 antibody

Images

  • Western blot analysis of ATBF1 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ER2001-49" style="font-weight: bold;text-decoration: underline;">ER2001-49</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ATBF1 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of ATBF1 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ER2001-49" style="font-weight: bold;text-decoration: underline;">ER2001-49</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of ATBF1 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-49, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"