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ASS1 Mouse Monoclonal Antibody [11F1]

Usd: 350

Specification

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Catalog# EM1701-87

ASS1 Mouse Monoclonal Antibody [11F1]

Application

  • WB

  • IHC-P

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

ASS1 Mouse Monoclonal Antibody [11F1]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human ASS1 aa 1-412 / 412.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IF-Cell, FC

Molecular Weight

Predicted band size: 47 kDa

Positive Control

HepG2, MCF-7, SiHa, human colon cancer tissue, mouse testis tissue.

Conjugation

unconjugated

Clone Number

11F1

RRID

AB_3068810

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2b

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:5,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.

Background References

1. Shaheen N et al. Characterization of human wild-type and mutant argininosuccinate synthetase proteins expressed in bacterial cells. Enzyme Protein 48:251-264 (1994).

2. Berning C et al. Investigation of citrullinemia type I variants by in vitro expression studies. Hum Mutat 29:1222-1227 (2008).

Sequence Similarity

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Tissue Specificity

Expressed in adult liver.

Post-translational Modification

Acetylated by CLOCK in a circadian manner which negatively regulates its enzyme activity. Deacetylated by histone deacetylases.

Subcellular Location

Cytosol.

UNIPROT

SwissProt: P00966 Human

SwissProt: P16460 Mouse

Synonyms

Argininosuccinate synthase 1 antibody

Argininosuccinate synthase antibody

Argininosuccinate synthetase 1 antibody

ASS antibody

Ass-1 antibody

ass1 antibody

ASSA antibody

ASSY_HUMAN antibody

Citrulline aspartate ligase antibody

Citrulline--aspartate ligase antibody

Expand

Images

  • Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (<a href="/products/EM1701-87" style="font-weight: bold;text-decoration: underline;">EM1701-87</a>) at 1/5,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: SiHa cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 47 kDa<br />Observed band size: 47 kDa<br /><br />Exposure time: 30 seconds;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1701-87" style="font-weight: bold;text-decoration: underline;">EM1701-87</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (EM1701-87) at 1/5,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: SiHa cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

    Exposure time: 30 seconds;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-87) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (<a href="/products/EM1701-87" style="font-weight: bold;text-decoration: underline;">EM1701-87</a>) at 1/2,000 dilution.<br /><br />Lane 1: A549-WT cell lysate<br />Lane 2: A549-KD ASS1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 47 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 40 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1701-87" style="font-weight: bold;text-decoration: underline;">EM1701-87</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (EM1701-87) at 1/2,000 dilution.

    Lane 1: A549-WT cell lysate
    Lane 2: A549-KD ASS1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 47 kDa
    Observed band size: 45 kDa

    Exposure time: 40 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-87) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ICC staining ASS1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining ASS1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining ASS1 (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining ASS1 (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining ASS1 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining ASS1 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ASS1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ASS1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASS1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASS1 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"