ALDH4A1 Mouse Monoclonal Antibody [7E3]
Catalog# EM1701-75
ALDH4A1 Mouse Monoclonal Antibody [7E3]
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WB
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IHC-P
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Human
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Rat
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unconjugated
Overview
Product Name
ALDH4A1 Mouse Monoclonal Antibody [7E3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within Human ALDH4A1 aa 514-563 / 563.
Species Reactivity
Human, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 62 kDa
Positive Control
SK-Br-3 cell lysate, HepG2 cell lysate, rat liver tissue, human liver tissue, human colon cancer tissue, human kidney tissue.
Conjugation
unconjugated
Clone Number
7E3
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IHC-P
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1:50-1:400
Target
Function
Aldehyde dehydrogenases (ALDHs) mediate NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde, lipid peroxidation and metabolism of corticosteroids, biogenic amines and neurotransmitters. ALDH4A1 (aldehyde dehydrogenase 4 family member A1), also known as P5CD (Delta-1-pyrroline-5-carboxylate dehydrogenase), P5CDh, P5CDhL, P5CDhS or ALDH4, is a major enzyme involved in the proline degradation pathway. Localizing to the mitochondrial matrix, ALDH4A1 catalyzes the conversion of Delta-1-pyrroline-5-carboxylate (P5C) to glutamate. A mutation in the gene encoding ALDH4A1 results in HPII (hyperprolinemia type II), a disease characterized by an excess of P5C and proline that is associated with mental retardation and seizures.
Background References
1. Srivastava D et al. The three-dimensional structural basis of type II hyperprolinemia. J Mol Biol 420:176-189 (2012).
2. Hochstrasser D F et al. Human liver protein map: a reference database established by microsequencing and gel comparison. Electrophoresis 13:992-1001 (1992).
Sequence Similarity
Belongs to the aldehyde dehydrogenase family.
Tissue Specificity
Highest expression is found in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas.
Subcellular Location
Mitochondrion.
Synonyms
AL4A1_HUMAN antibody
aldehyde dehydrogenase 4 antibody
aldehyde dehydrogenase 4 family, member A1 antibody
Aldehyde dehydrogenase family 4 member A1 antibody
Aldehyde dehydrogenase, family 4, subfamily A, member 1 antibody
ALDH4 antibody
aldh4a1 antibody
Delta 1 pyrroline 5 carboxylate dehydrogenase, mitochondrial antibody
Delta-1-pyrroline-5-carboxylate dehydrogenase antibody
L-glutamate gamma-semialdehyde dehydrogenase antibody
ExpandImages
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Western blot analysis of ALDH4A1 on different lysates with Mouse anti-ALDH4A1 antibody (EM1701-75) at 1/1,000 dilution.
Lane 1: SK-Br-3 cell lysate
Lane 2: HepG2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-75) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-ALDH4A1 antibody (EM1701-75) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-75) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ALDH4A1 antibody (EM1701-75) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-75) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ALDH4A1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-ALDH4A1 antibody (EM1701-75) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-75) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"