ACY-1 Rabbit Polyclonal Antibody
Catalog# ER1803-75
ACY-1 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
ACY-1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human ACY-1 aa 50-250.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 46 kDa
Positive Control
Mouse liver tissue lysate, Mouse kidney tissue lysate, Rat liver tissue lysate, Rat kidney tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:1,000
Target
Function
This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene.
Background References
1. Lindner H A et al. Essential roles of zinc ligation and enzyme dimerization for catalysis in the aminoacylase-1/M20 family. J Biol Chem 278:44496-44504 (2003).
2. Van Coster R N et al. Aminoacylase I deficiency: a novel inborn error of metabolism. Biochem Biophys Res Commun 338:1322-1326 (2005).
Sequence Similarity
Belongs to the peptidase M20A family.
Tissue Specificity
Expression is highest in kidney, strong in brain and weaker in placenta and spleen.
Subcellular Location
Cytoplasm.
Synonyms
ACY 1 antibody
ACY-1 antibody
Acy1 antibody
ACY1_HUMAN antibody
ACY1D antibody
ACYLASE antibody
Acylase I antibody
Aminoacylase 1 antibody
Aminoacylase-1 antibody
EC 3.5.1.14 antibody
ExpandImages
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Western blot analysis of ACY-1 on different lysates with Rabbit anti-ACY-1 antibody (ER1803-75) at 1/2,000 dilution.
Lane 1: Mouse liver tissue lysate (40 µg/Lane)
Lane 2: Mouse kidney tissue lysate (40 µg/Lane)
Lane 3: Rat liver tissue lysate (40 µg/Lane)
Lane 4: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-75) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ACY-1 antibody (ER1803-75) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-75) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ACY-1 antibody (ER1803-75) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-75) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-ACY-1 antibody (ER1803-75) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-75) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
ACY1 Mouse Monoclonal Antibody [15G2]
Application: WB,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
