Skip to content

ABCA1 Rabbit Polyclonal Antibody

Usd: 315

Specification

Bulk Order Quote | Customization Request

Catalog# R1510-42

ABCA1 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

ABCA1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human ABCA1 aa 1,101-1,312.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 254 kDa

Positive Control

HepG2 cell lysate, U-87 MG cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate.

Conjugation

unconjugated

RRID

AB_3073351

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

Target

Function

ABC1 (for ATP-binding cassette transporter 1) is a member of the family of ATP-binding cassette proteins which transport various molecules across biological membranes. ABC1 contains two characteristic ATP-binding domains and 12 transmembrane domains which form a channel-like structure for transport. Mutations in the ABC1 gene are implicated in Tangier disease, characterized by low serum high density lipoprotein. ABC1 is widely expressed in human tissues, with high levels of expression in liver, lung, adrenal glands, placenta and fetal tissue. ABC1 expression is induced during monocyte differentiation and upregulated in the presence of acetylated low-density lipoprotein. ABC1 may have a dual regulatory function in macrophage lipid metabolism and inflammation.

Background References

1. Mostafa, A.M. et al. 2015. Glucagon-like peptide 1 (GLP-1)-based therapy upregulates LXR-ABCA1/ABCG1 cascade in adipocytes. Biochemical and biophysical research communications.

2. Colla o, Rde C. et al. 2012. Protection by Mikania laevigata (guaco) extract against the toxicity of Philodryas olfersii snake venom. Toxicon. 60: 614-622.

Sequence Similarity

Belongs to the ABC transporter superfamily. ABCA family.

Tissue Specificity

Widely expressed, but most abundant in macrophages.

Post-translational Modification

Phosphorylation on Ser-2054 regulates phospholipid efflux.; Palmitoylation by DHHC8 is essential for membrane localization.

Subcellular Location

Cell membrane, Endosome.

UNIPROT

SwissProt: O95477 Human

SwissProt: P41233 Mouse

SwissProt: Q80ZB2 Rat

Synonyms

ABC 1 antibody

ABC Transporter 1 antibody

ABC-1 antibody

ABC1 antibody

ABCA 1 antibody

ABCA1 antibody

ABCA1_HUMAN antibody

ATP binding Cassette 1 antibody

ATP binding cassette sub family A ABC1 member 1 antibody

ATP binding cassette sub family A member 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of ABCA1 on different lysates with Rabbit anti-ABCA1 antibody (<a href="/products/R1510-42" style="font-weight: bold;text-decoration: underline;">R1510-42</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: U-87 MG cell lysate (20 µg/Lane)<br />Lane 3: SH-SY5Y cell lysate (negative) (20 µg/Lane)<br />Lane 4: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 5: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 254 kDa<br />Observed band size: 254 kDa<br /><br />Exposure time: 15 seconds; ECL: K1801;<br /><br />3-8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/R1510-42" style="font-weight: bold;text-decoration: underline;">R1510-42</a>) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of ABCA1 on different lysates with Rabbit anti-ABCA1 antibody (R1510-42) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: U-87 MG cell lysate (20 µg/Lane)
    Lane 3: SH-SY5Y cell lysate (negative) (20 µg/Lane)
    Lane 4: Mouse liver tissue lysate (40 µg/Lane)
    Lane 5: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 254 kDa
    Observed band size: 254 kDa

    Exposure time: 15 seconds; ECL: K1801;

    3-8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-42) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

ABCA1 Mouse Monoclonal Antibody [4G2]

Application: WB,IP,IF,IHC-P

Reactivity: Human,Mouse

Conjugate: unconjugated