Founded in 2007, HUABIO has over 15 years of experience in the antibody industry and was one of the first to capitalize on the advantages of recombinant antibodies. Although our products were sold under third party distributors, our antibodies gained the reputation of being the most accurate, efficient and consistent.
In 2020 we decided to stop selling our antibodies to other companies and instead work towards brand recognition and loyalty. Our company mission is still the same: to disrupt an already overcrowded antibody market with distinguishably reliable products. We're still dedicated to manufacturing and testing every product in-house before sending it directly to your lab.
So how do we ensure our antibodies stand out and perform consistently?
HUABIO'S 5 + 1 BLUEPRINT FOR REPRODUCIBILITY
Genetics
Knockout or knockdown the target gene by using CRISPR/Cas9 or RNAi technology in cells or tissues, and validate the antibody specificity by measuring the absence or reduction of the antibody signal in these cells or tissues.
Biological Properties and Orthogonal Data
Qualitative and quantitative verification based on biological properties, or use antibody-independent assays to detect correlations between antibodies and biological properties or non-antibody quantification.
Heterologous Expression
Antibody signal is evaluated in cell lines following heterologous expression of native (or mutated) target protein.
Multiple Antibodies
Validate by using two (or more) independent antibodies that can recognize different epitopes of the target protein.
Complementary Validation
Antibody specificity may be validated using complementation assays. Including competitive ELISA, peptide blocking, or protein arrays.
Recombinant Production
Recombinant production provides researchers with crucial advantages which include superior lot-to-lot consistency, continuous supply, and amenability to antibody engineering.


